SB/T 10389-2004 Determination of sorbic acid in meat and meat products

This standard specifies the determination method of sorbic acid in meat and meat products. This standard is applicable to the inspection of sorbic acid in meat and meat products. SB/T 10389-2004 Determination of sorbic acid in meat and meat products SB/T10389-2004 Standard download decompression password: www.bzxz.net

ICS 67.120
Preparation No.: 14170—2004Www.bzxZ.net
National Trade Industry Standard of the People's Republic of China SB/T10389—2004
Determination of Sorhic Acid in Nicotine and Meat Products
Determination of Sorhic Acid in Nicotine and Meat Products2004-08-04Promulgated
Ministry of Commerce of the People's Republic of China
Implementation on 2004-09-01
Chinese Standard SB/T10389—2004
National Trade Industry Standard of the People's Republic of China SB/T10389—2004Determination of Sorhic Acid in Nicotine and Meat Products2004-08-04Promulgated
Ministry of Commerce of the People's Republic of China
Implementation on 2004-09-01Chinese Standard SB/T10389—2004
This standard is initiated by: Jin Shesheng, Yang Wangmian, Song Shufeng, Han Huixin, Zhang Xinleng, Liu Pitu and entrusted to the Technical Supervision Center of the Ministry of Industry and Information Technology for analysis. 1 Scope
Determination of the content of meat and meat products
This standard specifies the determination of the content of meat and meat products by the kinetic method. This standard is applicable to the inspection of the content of meat and meat products by acid: 2 Principle
The sample is extracted and purified with neutral aluminum and then determined by high efficiency concentrated phase chromatography. 3 Test materials and materials
The following test materials are used: Unless otherwise specified, the analytical paper is used. Water is used as the medium. 3.1 Methanol: pure
3.2 Ammonium acetate,
3.3 Neutral oxidizing agent: (-00--200) 3.41, 12 mul/T. Acetic acid is concentrated; 1 tbsp. 1 tbsp. 3.5 Sodium carbonate: 99% (National Center for Standard Materials Research). 5B/110389-2002
3.6 Aldehyde standard stock solution: accurately take the standard sample, add a small amount of ethanol, and make up to 25 ml with water. The solution contains 1 tbsp. UmB. The shelf life is 15 days. 3.7 Standard solution of lysine: Pipette 5 μl of lysine standard stock solution into a 1 (2 mL) bottle, add water to dilute to the mark [9, then get lysine. Refrigerator: Available for 3 days. 3.B Standard solution of lysine: Collect 1.0, 2.0, 3.3.4.0, 5.0 ml of lysine standard solution respectively. Add water to the mark [0 mL] of each solution. Then get a standard solution containing 5.0, 10.0, 3.0, 20.0, 25 g lysine. 4 Instruments and equipment
4.1 High performance liquid chromatography: UV detector only; 4.2 Ultrasonicator.
4. 3 Rotary flowmeter,
4.4 Centrifugal flow,
4.5 Rotary flowmeter
4.6 Microporous energy filter.
5 Measurement steps
5. 1 Sample treatment
Table 2: Sample weight 1.0 ... mm, particle size: pm1SB/T10389—2004
a) mobile phase: 0.mol/L + formaldehyde = wV-: flow rate: 1.cm1.mia;
detection wavelength, 23(m:
5.2.2 Preparation of sorbic acid standard curve
Add 2% sorbic acid standard series dilution under the above chromatographic conditions. The peak area corresponding to the concentration of the concave carrier standard culture was used as the marker.
5.2.3 Sample identification
Under the above chromatographic conditions, accurately aspirate 2) and select the drop pool for HPLC separation. 6 Results 6.1 Calculation Analyze the accuracy of the standard curve points and the corresponding peaks, and then calculate the content of benzyl alcohol in the test sample according to the formula (XX1000). The unit is gram per kg of test liquid. When the standard line is accurate, the unit is gram per litre (m). Generally, the total volume of the test liquid is litre (m)1.
The mass of the sample is g.
6.2 Detection limit The detection limit of this method is 20:ml. When the sampling volume is less than 1g, the minimum detection limit is 2.3mg:≤g7. It is allowed that the same analytical results are determined simultaneously or successively.
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