GB/T 14926.28-2001 Method for detecting mouse parvovirus in experimental animals

This standard specifies the detection method and reagents for mouse parvovirus (MVM). This standard is applicable to the detection of mouse MVM. GB/T 14926.28-2001 Detection method for mouse parvovirus in laboratory animals GB/T14926.28-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T 14926.28-2001
Laboratory AnimalswwW.bzxz.Net
Microbiological Examination Methods (4)
Laboratory AnimalsMicrobiological Examination Methods2001-08-29 Issued
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine
2002-05-01 Implementation
GB/T14926.282001
This standard is a revision of GB/T14926.28—19948 Laboratory Animals Mouse Parvovirus Examination Method. No changes have been made to the examination method. Only some of the original text has been modified. This standard was proposed and managed by the Ministry of Science and Technology of the People's Republic of China. The drafting unit of this standard is the Chinese Society of Laboratory Animal Science. The main drafter of this standard is Zhongqin.
This standard was first issued in January 1994.
National Standard of the People's Republic of China
Laboratory animal
Test method for minute wirus of mice
Laboratory animalMethod for examination of minute wirus of mice (MWM)
This standard specifies the test method and reagents for minute wirus of mice (MVM). This standard is applicable to the test of MVM of mice
Cited standards
GB/T 14926.282001
Reference GB/T14926-28—1994
The provisions contained in the following standards constitute the provisions of this standard by citing them in this standard. When this standard is published, the versions shown are valid. All standards will be revised. Parties using this standard should explore the possibility of using the latest versions of the following standards: GB/T14926.50—2001
GB/T14926.51—2001
GB/T14926.52-2001
Laboratory animals
Laboratory animals
Laboratory animals
Alcohol-linked immunosorbent assay
Immunoenzyme assay
Immunofluorescence assay
According to the principles of immunology, MVM antigen is used to detect MVM antibodies in mouse serum. Main reagents and equipment
4.1 Reagents:
4.1.1 ELISA antigens
41.1-1 Specific antigens
MVM inoculated with mouse renal cell (ME) or 3T3 cells, maintained and cultured for 7~10 days, harvested when the cell lesions reached +++~++++. Freeze for three times or ultrasonic treatment, centrifuged at low speed to remove the lesions, and the supernatant was concentrated by ultracentrifugation to make ELISA antigens
4-1-1-2 Positive antigens
ME or 3T3 cells were freeze-thawed, and the supernatant was removed by low speed centrifugation to remove the lesions. 4-1.2 Antigen slices
MVM inoculated with rat renal cell (RE) or ME cells, cultured for 7~10 days. When the lesions reached +++~++++, they were digested and dispersed with trypsin, washed with PBS, and smeared. After drying at room temperature, they were fixed with cold acetone for 10 minutes and stored at -20°C. 4.1.3 Positive serum
Antiserum obtained by immunizing SPF mice with MVM antigen 4.1-4
Negative serum
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on 2001-08-29 32
Hot sale on 2002-05-01
SPE mouse serum:
GB/T14926-28—2001
Enzyme conjugate
Horseradish peroxidase-labeled sheep or rabbit anti-mouse IgG antibody: or horseradish peroxidase-labeled Staphylococcus protein A (SPA) isothiocyanate-labeled sheep or rabbit anti-mouse IgG antibody, 4.1.6
42 Equipment
Fake label,
Fluorescent star microscope.
Yutong microscope,
37C tower culture box or water filling box,
Detection method
Use ELISA method (see GB/T14926-50-2001) for serological test. Use IFA method (see GB/T14926.52-2001) for serological test. Use IEA method (see GB/T14926.51-2001) for serological test. Result judgment
For positive test results, use the same method or another method to try again. If it is still positive, it is judged as positive. Result report
Make a report based on the judgment result.
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