GB/T 5413.26-1997 Determination of taurine in infant formula and milk powder

This standard specifies the method for the determination of taurine by high pressure liquid chromatography. This standard is applicable to the determination of taurine in infant formula and milk powder. GB/T 5413.26-1997 Determination of taurine in infant formula and milk powder GB/T5413.26-1997 Standard download decompression password: www.bzxz.net

GB/T5413.26—1997
The post-column derivatization ion chromatography method for determining taurine given in this standard was developed after repeated experiments and verification based on the search of 21 foreign literatures in the past 10 years. The recovery rate of this method is 100%, and the coefficient of variation is 2.19%. This series of standards will replace GB5413-85 from the date of implementation. This standard is proposed by the China Light Industry General Association.
This standard is under the jurisdiction of the National Dairy Standardization Center. The responsible drafting unit of this standard: Zhejiang Light Industry Research Institute. The participating drafting units of this standard: Food Hygiene Supervision and Inspection Institute of the Ministry of Health, National Dairy Product Quality Supervision and Inspection Center, Harbin Morinaga Dairy Co., Ltd., Nestle (China) Investment Services Co., Ltd. The main drafters of this standard: Ren Yiping, Huang Baifen, Chen Qingjun. 327
National Standard of the People's Republic of China
Infant formula and milk powder
Determination of taurine
Milk powder and formula foods for infant and young children-Determination of taurine content1Scope
This standard specifies the method for the determination of taurine by high pressure liquid chromatography. This standard applies to the determination of taurine in infant formula and milk powder. 2Method Summary
GB/T 5413. 26-1997
Replaces GB5413-85
The sample is dissolved in metaphosphoric acid solution, extracted by ultrasonic oscillation, centrifuged, filtered by microporous membrane, separated by sodium ion chromatographic column, and then derivatized with o-phthalaldehyde (OPA) and detected by fluorescence detector for quantification. 3Reagents
All reagents, if no specifications are specified, are analytically pure; all experimental water, if no other requirements are specified, is grade tertiary water. 3.1 Metaphosphoric acid solution: c(H.PO,) is 10g/L. Weigh 10.0g metaphosphoric acid and dissolve it in 1000mL distilled water. 3.2 Mobile phase: Weigh 19.6g trisodium citrate, dissolve it in 950mL water, add 1mL phenol, adjust the pH value to 3.10~~3.25 with nitric acid with H+ concentration of 6mol/L, and filter through a 0.45um microporous filter membrane. 3.3 Post-column fluorescence generation reaction reagent
3.3.1 Potassium borate solution: cK, BO.) is 0.5mol/L. Weigh 123.6g boric acid and 105g potassium hydroxide, dissolve and adjust the volume to 4L. 3.3.2 OPA solution: Weigh 0.60 g of OPA, dissolve it in 10 mL of methanol, add 0.5 mL of 2-mercaptoethanol and 0.35 g of Brij-35, add 0.5 mol/L potassium borate solution (3.3.1) to 1000 mL, and filter through a 0.45 um microporous membrane.
3.4 ​​Standard solution: The concentration of taurine is 10 μg/mL. Accurately weigh 0.100 g of taurine standard, dilute to 100 mL with water, then pipette 1.0 mL of this solution to 100 mL, and filter through a 0.3 um microporous membrane after diluting. 4 Instruments
Common laboratory instruments and:
4.1 Separation column: Sodium ion amino acid separation column. 4.2
Post-column reactor: Amino acid post-column reactor. 4.3 Fluorescence detector.
4.4 Solvent pump: flow rate is 0.01~0.09mL/min. 4.5 Data processing system: integrator.
4.6 Ultrasonic oscillator.
Approved by the State Administration of Technical Supervision on May 28, 1997 328
Implemented on September 1, 1998
5 Operating steps
GB/T5413.26--1997
5.1 Accurately weigh 1.0~~5.0g of sample (the content of taurine in the weighed sample is not less than 5μg), dissolve the sample in 60mL metaphosphoric acid solution (3.1), and transfer it into a 100mL volumetric flask.
5.2 After the sample solution is fully stirred, put it into an ultrasonic oscillator for 10~15min, and after cooling to room temperature, dilute to the scale. 5.3 Take 60mL of the sample solution after shaking and centrifuge at 5000r/min for 20min. 5.4 Take 10mL of the sample solution (supernatant) after centrifugation, filter it through a 0.3um microporous membrane, and take about 1mL of the intermediate filtrate for injection. 5.5 Chromatographic conditions.
5.5.1 Mobile phase flow rate: 0.3mL/min. 5.5.2 Column temperature: 55℃.
5.5.3 OPA derivatization reagent flow rate: 0.3mL/min. 5.5.4 Fluorescence detector: excitation wavelength: 338mm, emission wavelength: 425mm, sensitivity: 0.01AUFS. 6 Expression of analysis results bzxz.net
Taurine content in sample (mg/100g) = ×.100 × 100m X 1000
Where: c--concentration of the sample solution measured by the instrument, μg/mL, mass of the sample, g.
7 Tolerance
The difference between two measured values ​​of the same sample shall not exceed 5% of the average value of the two measurements. (1)
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