GB/T 14926.22-2001 Detection method for mouse hepatitis virus in experimental animals

This standard specifies the detection method and reagents for mouse hepatitis virus (MHV). This standard is applicable to the detection of mouse MHV. GB/T 14926.22-2001 Detection method for mouse hepatitis virus in experimental animals GB/T14926.22-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T14926.22-2001
Laboratory Animals
Microbiological Examination Methods (4)
Laboratory AnimalsMicrobiological Examination Methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
CB/T14926-22-2001
This standard is a revision of GB14926.22-1994 Laboratory Animals Mouse Hepatitis Virus Examination Method, adding the immunofluorescence test method. Some words in GB/T14926-22-1994 have been deleted and modified. This standard is proposed and managed by the Ministry of Materials Science and Technology of the People's Republic of China. The drafting unit of this standard is the Chinese Society of Laboratory Animal Science. The main drafter of this standard is Qu Duqin.
This standard was issued in January 1994.
National Standard of the People's Republic of China
Laboratory animal-Method for examination ofmouse hepatitis virus
Laboratory animal-Method for examination ofmouse hepatitis virus (MHV) This standard specifies the following contents: 1. This standard is applicable to the following standards: 2. Reference standards: The following standards are generally used in the inspection scope and are valid through the adoption of the standards. All standards have been revised and use GB/T149 GB/T149 GB/T148 3. Principle: 2001 Experimental scope: Experimental animals: Based on immunology, use MHV antibodies 4. Main test methods and instruments 4.1 Agents: 4.1 .1 ELISA antigen composition is the basis of this paper. Jinle uses immunofluorescence assay to detect mouse blood. 4.1.1.7 Specific antigens MHV (including MHVAMV-ASS) GB/T14926.22-2001 replaces the provisions of GB/T14926-22-1994. When the standard is published, the versions shown are the latest versions of the standard. Each strain of acute infection or L929 cells, 2% of the lesions after 4 days of inoculation, harvest, room temperature three times or ultrasound after reconstruction , remove cell debris by low-speed centrifugation, and the supernatant is concentrated by high-speed centrifugation to make ELTSA antigen
4.1-1-2 Normal antigen
DBT or L929 cells are frozen and thawed, and the supernatant obtained by low-speed centrifugation to remove cell debris, 41-2 Antigen
MHV infection nest DBT or L929 cells are inoculated for 1~2 days. When the lesions reach 10~10+10, they are digested and dispersed with lysate, washed with PBS, removed, dried in room temperature, fixed with cold acetone for 10 minutes, and stored at -20℃4.1-3 Positive electroporation
MHV antigen immune cleansing antiserum obtained from SPF mice, 4-1-4 Antigenic seat slide| |tt||General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China 2001-08-29 Approval 2002-05-01 Screening
Clear or SPF mouse serum,
4.1.5 Alcohol conjugate
GB/T14926.222001
Rattan root peroxidase labeled sheep or rabbit anti-mouse IgG antibody: or root peroxidase labeled mycotoxin protein A (SPA). 4.1-6 Isocyanate fluorescein labeled sheep or rabbit anti-mouse IgG antibody. 2 Materials
4.21 Microplate reader.
42.2 Fluorescence microscope.
42.3 Ordinary microscope.
4.2.437C network culture box or water submersible box.
5 Detection method
5-1 ELISA method (
5.2 IFA method (
5.3 IEA method
Result judgment
For positive detection
sperm result report
According to the material determination museum
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