GB/T 14926.30-2001 Detection method for rotavirus in experimental animals - rabbit

GB/T 14926.30-2001 Laboratory Animals Rabbit Rotavirus Detection Method GB/T14926.30-2001 Standard Download Decompression Password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T 14926.30-2001
Laboratory Animals
Microbiological Examination Methods (4)
Laboratory AnimalsMicrobiological Examination Methods2001-08-29 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T14926.30-2001
Rotavirus Immunization Examination Methods. No changes have been made to the examination methods. This standard is a revision of GB/T14926.30-1994 Laboratory Animals. The wording of the relevant contents of the original standard has been revised. This standard was proposed by the Ministry of Science and Technology of the People's Republic of China and is under the jurisdiction of the Chinese Society of Laboratory Animal Science. The main drafter of this standard is He Zhengming.
This standard was first issued in January 1994.
1 Scope
National Standard of the People's Republic of ChinaWww.bzxZ.net
Laboratory animai-Method for esamination of rabbit rolawrus (RRV)
This standard specifies the detection of GCRRV
This standard is applicable to the detection of RRY
2 Referenced standards
The following standards are valid. All standards
GB/T1492
new text, through
Phi Health Order·Use non
2001 Laboratory Animals
GB/T1492
GB/T1492-2001 Dangerous Animals
3 Principle
Detection of rotavirus
antigen in immune serum
4 Main test methods and equipment
4.1 Trial production
4.1.1 ELISA antibody
4.1.1.1 Specific antigen
D and RRV The antigen of the test sample of the joint immune variable absorption test product of Huang Guanglin is GB/T14926.30-2001, GB/T14926-30-1994, and the original version is approved for publication. The versions shown are all marked with the possibility of the new version. The principle of ELISA is to inoculate M%104 cells with rotavirus (SA11 strain) and monkey rotavirus (SA11 strain), and harvest them when the cells reach +++~++++ after 2~3 days of inoculation. After three times of thawing or ultrasonic treatment, the cell supernatant is removed by low-concentration centrifugation and then made into ELISA antigen by ultrasonic centrifugation. 4.1.1.2 Normal antigen
MA-104 fat was frozen and crushed, and the supernatant obtained by low-speed centrifugation to remove the fat fragments. 4.1.2 Antigen fragment
MA-104 cells were infected with polymorphism of rotavirus (SA11 strain). After 23 days, the cells were digested with lysate and dispersed, washed with PBS, and the fragments were removed. After drying at room temperature, they were fixed with cold acetone for 10 minutes and stored at -20℃. 4.1.3 Positive serum
Antiserum obtained from immune animals immunized with polymorphism of rotavirus (SA11 strain): or serum from immune animals recovered from infection. 4.1.4 Antisera
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 2001 and implemented on May 1, 2002
Antiserum of immune animals without rotavirus infection program.
4.1.5 Enzyme conjugate
GB/T 14926.30-2001
Horseradish peroxidase labeled goat anti-rabbit IgG antibody: or horseradish peroxidase labeled Staphylococcus protein A (SPA) 4.1-6 Heterochromic acid fluorescein labeled goat anti-rabbit IgG antibody 42H
4.2.1 Down-concentration instrument.
4.2.2 Fluorescence microscopy.
4.2-3 Ordinary microscopy.
4.2.437C Cultivation box or water hose box
Detection method
51 If the result is positive, please use the ELISA method
5.2 If the result is positive, please use the IFA method
Use the positive A
Result identification
For positive test results, select a method
Result report
Make a report based on the judgment department.
If the result is positive, it is judged as positive
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