QB/T 2407-1998 Determination of D-panthenol content in cosmetics

This standard specifies the ultraviolet spectrophotometric method for the determination of D-panthenol content in cosmetics. This standard is applicable to the determination of D-panthenol in cosmetics. When this method has interference, it is recommended to use high performance liquid chromatography for determination. The minimum limit for quantitative detection of D-panthenol in this standard is 0.03%. QB/T 2407-1998 Determination of D-panthenol content in cosmetics QB/T2407-1998 Standard download decompression password: www.bzxz.net

QB/T 2407-- 1998
This standard is prepared in accordance with GB/T1.1-1993 "Guidelines for Standardization Work Part 1: Basic Regulations for the Preparation of Standards". This standard is based on the principle that D-panthenol reacts with fluorescamine after hydrolysis to generate a strong fluorescent derivative, which can be measured by spectrophotometer.
This standard is proposed by the Industry Management Department of the State Bureau of Light Industry. This standard is under the jurisdiction of the National Cosmetics Standardization Center. The drafting unit of this standard: Shanghai Daily Chemical Research Institute. The main drafters of this standard: Lin Yan, Han Meifang, Gan Pingping. 102
1 Scope
Light Industry Industry Standard of the People's Republic of China
Determination of D-panthenol content in cosmetics
This standard specifies the ultraviolet spectrophotometric method for the determination of D-panthenol content in cosmetics. QB/T2407—1998
This standard applies to the determination of D-panthenol in cosmetics. When this method has interference, it is recommended to use high performance liquid chromatography for determination. The minimum limit for quantitative detection of D-panthenol in this standard is 0.03%. 2 Referenced standards
The provisions contained in the following standards constitute the provisions of this standard through reference in this standard. When this standard is published, the versions shown are valid. All standards will be revised, and parties using this standard should explore the possibility of using the latest version of the following standards. GB/T8170—1987 Numerical rounding rules
3 Principle
D-panthenol will hydrolyze with acid at a certain temperature, and the hydrolysis product will react with fluoresceinamine in a medium with a pH of 8 to 9 to generate a highly fluorescent derivative. The complex can be determined by a visible spectrophotometer with an absorption wavelength of 380nm to 390nm. 4 Reagents
Analytical grade (AR) reagents should be used in the analysis, and distilled water or water of equivalent purity should be used for the test. 4.1 Fluorescamine-acetonitrile solution 0.4mg/mL Accurately weigh 0.0400g of fluorescamine and add a small amount of acetonitrile to a beaker, transfer to a 100mL volumetric flask, dilute to the mark with acetonitrile, shake well, and set aside. 4.2 Borate buffer solution 0.6mol/L Weigh about 35g of boric acid, dilute to 1L with distilled water, and adjust the pH to 8.0±0.1 with 2mol/L sodium hydroxide solution on a pH meter. 4.3 Hydrochloric acid (GB/T622) 0.5mol/L ethanol solution Transfer 42mL of concentrated hydrochloric acid to 1L of 95% ethanol to obtain 0.5mol/L hydrochloric acid-acetonitrile solution. 4.4 D-panthenol standard solution 0.2mg/mL
Accurately weigh 2.000g of D-panthenol (dried) and dilute to 100mL with 95% ethanol. After shaking, pipette 10mL into a 100mL test tube, add 80mL of 0.5mol/L hydrochloric acid-ethanol solution, and heat in a water bath at (85±2)℃ for 30min. Take out and cool, transfer to a 100mL volumetric flask, and dilute to scale with 95% ethanol. Transfer 25mL of the above solution into a 250mL volumetric flask, and dilute to 250mL with 95% ethanol
5 Instruments
5.1 Spectrophotometer: wavelength range 200nm～500nm. 5.2 Constant temperature water bath sugar.
5.3 pH meter: graduation value 0.02.
5.4 Volumetric flask: 100, 250, 1000mL. 5.5 Beaker: 100, 250mL.
Approved by the State Bureau of Light Industry on November 25, 1998
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Implemented on June 1, 1999
5.6 Pipette: 1, 2.25mL.
5.7 Graduated test tube: 10, 100ml.
5.8 Analytical balance: graduation value 0.0001g. 6 Determination steps
6.1 Drawing of standard curve
QB/T 2407---1998
Accurately pipette 0, 1, 2, 3, 4mL of D-panthenol standard solution into a 10mL graduated test tube (equivalent to 0.0, 0.2, 0.40.6, 0.8mg), respectively, pipette into 4mL of fluoramine solution, and dilute to 10mL mark with saline buffer. Shake well, measure the absorbance at 387nm with 1cm colorimetric III, and draw a standard curve of absorbance versus concentration. 6.2 Sample preparation
Accurately weigh 10g of sample (accurate to ±0.0002g, the sample contains about 1% D-panthenol), put it in a 100mL graduated test tube, and heat it in a water bath at about 80C for about 30min. After cooling, add 80ml of 0.5mol/L hydrochloric acid-ethanol. Then heat it in a (85±2)℃ water bath for 30min. After cooling, transfer it to a 100ml volumetric flask, make up to volume with 95% ethanol, shake it, if turbidity occurs, centrifuge it, and take out the clear solution for use.
6.3 Determination
Pipette 0.1mL~0.3mL of the test solution into a 10mL graduated test tube, and proceed as in 6.1. Calculate the D-panthenol content using the standard curve.
6.4 Expression of results
The content of D-panthenol in the sample is calculated according to formula (1): D-panthenol content (%) =
C×100/V
mx1000
Wherein:. The amount of D-panthenol obtained from the standard curve, mg; sample mass·g;
V The amount of test solution transferred, mL.
The obtained results are rounded to two decimal places according to GB/T8170. 7 PrecisionwwW.bzxz.Net
The difference between the results of two parallel determinations shall not exceed 10% of the average value. 10.1
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