Plant quarantine--Methods for inspection and identification on Khapra Beetle （Trogoderma granarium Everts）

This standard specifies the inspection and identification methods for Trogoderma granarium Evertsl. This standard applies to the quarantine and identification of Trogoderma granarium. GB/T 18087-2000 Plant quarantine Quarantine and identification methods for Trogoderma granarium GB/T18087-2000 Standard download decompression password: www.bzxz.net
This standard specifies the inspection and identification methods for Trogoderma granarium Evertsl. This standard applies to the quarantine and identification of Trogoderma granarium.

cx 65. 020
National Standard of the People's Republic of China
GE/T18087-2000
Plant quarantine
Plant guarantine-Methods for inspection and identification on Khapra Beetle (Trogoderma granarivm Everts) 2000- 04 - 26 Released
2000-10-01Implementation
National Quality and Technical Supervision Bureau Released
G3/T15087—2000
It is the inspection point for the dangerous insect pests imported into my country. In order to prevent the transmission of white corn spot to human beings, the goods imported should be monitored and the correct treatment of white corn spot should be followed. During the formulation process of this standard, relevant research results at home and abroad were considered, and the data were analyzed and implemented. The relevant characteristics of white corn spot can be used as the basis for the identification of white corn spot alone or at the same time. This standard is proposed by the Ministry of Agriculture:
This standard is issued by the Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The standard is a small book written by the Chinese people's government, and the standard is in the form of a Chinese version. The standard is a model of the People's Republic of China.
National Standard of the People's Republic of China
Plant Quarantine Quarantine Identification Methods'lant quarantine-Methods tor inspection and identificationnnKhapraBcetleTragoderragranarinmK'verls This standard specifies the inspection and identification methods of grain quality ferrrEves. The standard is applicable to the quarantine and identification of grain quality ferrrEves. 2 Principles
GBT 18087--2C0
2.1 Grain quality ferrrEves are not allowed to be transported by goods, packaging materials and transportation tools. 2.2 The distribution, morphological evidence, internal anatomical characteristics and characteristics of the corn spot provide the basis for the formulation of inspection and identification methods.
3 Dissolving assembly
3.110 Potassium hydroxide solution, add 1g potassium hydroxide and 30mL hot water and shake it: used for anatomical processing of specimens. 3.2 Formalin solution: 1 part formalin containing 40% formaldehyde and 17-19 parts sodium bicarbonate, used for the preservation of insects. 3.3 Alcohol solution: Add 1 part formalin containing 40% formaldehyde and 17-19 parts sodium bicarbonate to form a solution, used for the preservation of insects and larvae. 3.4 Calcein solution: 17 parts 95% alcohol plus 2 parts chlorinated formaldehyde, 6 parts warm, 28 parts hot and dry. Preserve specimens of the same species as the insect body morphology.
3.5 Sealing solution: Add 30 g of gum arabic into 0 ml of natural water. Place in a (5V~%) aqueous solution. After the amine is completely dissolved, add trichloroacetaldehyde and 2C1 oil. Use dry slides. 4 Instruments and utensils
4.1 Microscope, mirror. bzxZ.net
42 culture melon, small burning pivot, marker light, micro knife, dissecting needle, carrier slice, cover ring slice. Suction, small hair fan, cup stick, various aperture sample sieves 4.3 choose 11-zhong-8 hungry 14-e1-exdecc: anti-junction as: sex agent. 4.1 choose Zhilin tears, Yingzi Shan, Yanfa Shan, corn oil, Chongya sleeves, etc. as food attractants. 4.5 Select paper traps with sex attractants or lures containing new materials for inspection. 5.1.1 Spot check should be carried out on site. 5.1.2 Check randomly. 5.1.3 Check the efficiency of each piece: 0.5% of the total efficiency of the goods, 50W pieces or less (501~-100) pieces are sampled (11~3U pieces are sampled (11~20 pieces are sampled 31 pieces or more), 5K pieces are added, and 1 piece is slightly added (micro-loaded goods). Fujian Quality and Technical Supervision Bureau approved 2C0010-01 on April 25, 2000. 5.2 Sampling 5.2.1 On-site sampling station for joint inspection, GHT13037-2000 5.2.2 When checking easily sieved goods, sieve all the collected (1-3) items in each cargo, and bring 16 mixed samples (less than 1kg + 1kg) and sieved items together for indoor inspection. When checking non-grain goods, the sampling quantity is determined according to the conditions: the packaged goods are counted as 1 piece per 100kg. 5.3 Quarantine method 5.3.1 Screening inspection: for grains, beans and legumes 5.3.2 Inspection of meat: Packing materials, filling materials, cushion materials, containers, transportation tools, and animal products should be inspected visually. In particular, check the edges, corners, and places of sacks: wrinkles, edges, and gaps of bags and bags: corners and seams of paper: transportation tools, corners and seams of boards
5.3.3 Lure: Put sex lures, food lures, or both into the lure. Put the lure in the packaging room, transportation equipment, container warehouse, corners of the medicine chain, and other places, and collect the lure regularly. 5.3.1 The samples collected in accordance with 5.2 shall be placed in a 32~353% humidification box for observation. 5.4 Collected specimens: After checking the collected specimens, the worms, worms, molts and molts shall be stored in separate racks. The specimens shall be judged according to the determined method.
6 Laboratory identification
6.1 Main characteristics of the worm (Trgenmmr) 67.1 The epidermis is generally yellowish, with a red to yellow basal band, a middle band and a sub-terminal band on the short sheath, and the sub-terminal band is usually annular 61.2 The head has a single coat.
6.1.3 Antennae 11 City: Antennae are difficult to distinguish 3~3 segments, and the antennae of insects have 3~4 pads. 6.1.4 The first segment is longer than the second segment.
6.2 The main features of the antlers
6.2.1 Adults: Body length (1.8-3.0>mm1, (0.9--=.7a1) larger than males, head and front protuberance color to black. Red: Antennae of males with 5 segments, the end of the segment is 31, and the antennae are only 31; Antennae are wide and will be: Antennae are particularly degenerate: one-third of the total length is missing, with a deep center in the middle of the third of the total length, both round, the maximum height is not supported by the depth of the concave end. The bone pieces are short, about 0.2mm long, 0.01mm wide, and the upper part is returned to the Antennae: The teeth are few, 4.0-6.7)m long (1.4-2.5)m (average 1.5-6.2.7mm). Antennae are red to black, 2 sections of equal length, with setae on the first section borne around the section, and the first quarter without brushes on the outside. The first section has awns with bristles not exceeding the anterior renal groove. The inner part of the first section has 3-5 sections, while the female has 3-4 sections. The posterior width of the iris is degenerate and points upward. The remaining one-third of the total length disappears in females, and the front edge has a deep frontal margin in the middle. The lateral end of the face is small and can be determined as a disc. 7.2 If the insect has one protuberance on the upper inner lip and the grains produced on the 8th segment have no anterior groove, it can be identified as a grain spot capsule.
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