GB/T 5009.88-2003 Determination of insoluble dietary fiber in food

This standard specifies the neutral detergent determination method for insoluble dietary fiber in plant foods. This standard is applicable to the determination of insoluble dietary fiber in various plant foods and mixed foods containing plant foods. The detection limit of this method is 0.1 mg. GB/T 5009.88-2003 Determination of insoluble dietary fiber in food GB/T5009.88-2003 Standard download decompression password: www.bzxz.net

TCS 67.040
National Standard of the People's Republic of China
GB/T5009.88—2003
Generation GB12—
Determination of insoluble dielary fiber in food
foods2003-08-11 issued
Ministry of Health of the People's Republic of China
China National Standards Administration Committee
2004-01-01 implementation
GB/15009.88-2003
Standard 4 (04 985.29 Determination of total dietary fiber in foods-Main method, Standard ... GB/123391.421316 standard, regulation: Section 4: Chemical protection: The Ministry of Health of the People's Republic of China has revised the original standard. The Chinese Academy of Medical Sciences Nutrition and Food Biology Institute is responsible for the main bid. This standard is the first release, and this is the first time to subscribe. 3e
1 StandardwwW.bzxz.Net
Determination of non-organic dietary fiber in food
2 Standards for the determination of non-organic dietary fiber in food is the method of GA/T 5009.$8—2003
The standard advocates the use of plant materials as food and food containing plant materials to reduce the loss of food without increasing the amount of vitamins. The method is as follows: 9 steps
2 Principle
Under the digestion of neutral detergent, the starch, protein, and other substances in the sample are homogenized and decomposed to remove the indigestible residual insoluble fiber, mainly including fiber cord, organic fiber, cellulose, keratin, silicon dioxide, etc., and also includes insoluble ash. 3 Reagents
3.1 Anhydrous sulfuric acid.
3.2 Oil fermentation: boiling 3.30
3.3 Propanediol.
3.4 ​​Toluene.
3.5 Neutral washing solution: Put 13.61g sodium diethyl ether and 3.51g tetrahydrofuran in a 10% H2O solution in a cup. Add about 150mL of water and heat to dissolve. Put 30g of ethanol and 100ml of ethyl ether (chemically pure) in about 79ml of hot water, combine the above two solutions, then put 4,66g of anhydrous sodium disodium iodide in 1% hot water, discard and precipitate, and mix with phosphoric acid until H6. 9~~7. 1. Finally, add 3g.7nl of water to 1(ml). 3.6 tartrate solution: 3g.7nl/T. monophosphate and 61.3mI.0.1mol/L dihydrogen phosphate to make EI[7.0:
3.7, 2.a flow dregs: 10 parts (Sigma-Aldrich, USA, product number 6990-A, value: 1UJLII.U. Phosphate coagulant: commercial, filtered and set aside. 3.8 Heat the mixture (heat-resistant 130C, USA CArning, YREX brand. Other brands are also acceptable, as long as it is heat-resistant and does not break).
4 Receiver
4.1 Commonly used laboratory equipment:
4.2# extract, 115'C~10 years,
4.3 Box: 37℃+2%.
4.4, fiber measuring instrument,
4.5 If installed in the fiber measuring instrument, it can be composed of the following parts: 4.5.1 Electric heating plate: with temperature control device.
4.5.2 High-end non-drinking burning. 600ml..
4.5.3 Mother-type low-heat de-oiling device, the most dense mL. Aperture m~6Jm4.5.4 Flow cold spot device:
1) This information, the six steps and the approval of the product: if the effect between other equivalent products, then these products
GH/T500588-m20n3
4.5.5 Pull method to install the stomach, to pump. Pump method and water pump composition 5 analysis Steps
5.1 Sample processing
5.1.1 Cut the sample into pieces and bake them at 6u℃ for 3 times to remove the surface moisture, sieve them for 0-3 days, put them in a small plastic bottle, put a small bag of barrier bottle in it, and set aside. 5.2.2 For vegetables and other organic foods, take the edible part, rinse it with water for 2 times, then cut it into pieces with gauze. Weigh about 100% of the sample and calculate the moisture content. Sieve for 20-33 minutes and set aside. Before the test, use a cloth to absorb the drops, grind, mix and set aside. 5.2 Determination 5.2.1 Accurately weigh the test C.5g~1.00g blue type element in a clear beaker. When the effective fat content exceeds 13, it is necessary to remove the fat. Example 1.03, use oil fermentation (30-603 times 3m5.2.2 Add 9mL of detergent, and then return to the center, bg of water-free industry flow late sodium. 5.2.3 Add salt and heat it within 5min-10mi. Move it to the electric drying plate and keep it at 1l. 5.2.4 Heat-resistant amine glass filter: only ~3g glass cotton, dilute to the box, 111 plate 41, take out the stomach medium and cool it in the room. Remove, crazy proof (can break the silk number 4 digits). 5.2.5 Pour the whole hot test column into the filter ticket Use water system method, 1000ml hot water: -100, filter and filter, drain the residue, wash the lower part of the filter and soak it, cool it, 5.2.6 quickly add medium liquid, melt and limit the cotton, press the gas sea, add a few drops of Chinese medicine, and pass it through the temperature of 5.7.
6.2.7 Take out the filter, dry it, select the liquid, and use 3Uuml. hot water to wash away the residual liquid, check the starch residue, if there is any, add alcohol to hydrolyze, the starch has been removed, and then drain it twice. 2.2.9 Dry the filter at 110℃ for 4h, dry it, and weigh it to 4 decimal places.
6 Calculate the result
X- n= 1 x1
The content of non-abrasive fiber in the sample.%X
The mass of added sugar in the sample, in grams (g); the test results of the test results are only grams (
). The scoring results represent the minimum effective point.
? Precision
The absolute difference between the two results obtained under multiple tests shall not exceed % of the calculated average value. G3e
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