GB/T 14926.14-2001 Detection method of Staphylococcus aureus in experimental animals

This standard specifies the detection method of Staphylococcus aureus in experimental animals. This standard is applicable to the detection of Staphylococcus aureus in mice, rats, guinea pigs, hamsters and rabbits. GB/T 14926.14-2001 Detection method of Staphylococcus aureus in experimental animals GB/T14926.14-2001 Standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
B/T 14926.14-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T1492614-2001
This standard is a revision of GB/T14926.14—1994 (Test methods for Sheppardella aureus in laboratory animals) and GB/T14926.141954 (5.2 points for high-quality testing) The method of bacterial enrichment, in the use of direct technology of high salt culture medium to achieve high concentration, increased by 6.3, mannitol test positive This standard is reserved for the Ministry of Science and Technology of the People's Republic of China. The standard unit is China Experimental Animal Society. The main author of the standard is Huang Hua.
This standard was published in January 1994.
National Standard of the People's Republic of China
Detection method for Staphylococcus aureus
Laboratory animal
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Culture medium and test type
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CRRC National Quality Supervision and Inspection Police Hot Customer General 2001-08-29 approval document
GB/T14926-142001
approval E/T436.14—1994
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detection procedure
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7.1 sampling
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GE/T14926.14—2001
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the bacteria of the real blood
are collected from the ileocecal area or the clean area, and cultured
in a static culture medium with high salt content (35 ± 1% 1821, 7.3 Baoding
7.3.1 Surface characteristics
are generally cultured on a high salt culture medium in a sweet zone, with a height of about 1mm, and a yellowish white colony. The colony is formed by the red Anwei yellow special blood culture medium (36 ± 1% C) for 18-24h. The pattern is raised, the surface is smooth, and there is a blood ring around the pattern.
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GB/T14926-14—2001
Apple blue positive grass needs - arranged false bacteria sweet shape, no respect · no beauty waist straight 0～1.0pm7.3.3 mannitol hall alcohol test stable positive,
7.3.4 blood tight medical enzyme test
limit 1+4 fresh electric non-reading -20C as early as the free by packing 0.5mL put 1 small test, then mail people to check bacteria 4h Insert 0.BmL of meat culture medium into the culture box or water hose, observe every 30min overnight, and observe for 6h. At the same time, use known positive and resistant markers and free culture medium as controls. When the known positive strains and the to-be-tested templates all show the same or a little bit of love, it can be judged as positive.
8 Result report
Any test that fails to meet the requirements will be considered as a positive report, and no positive report is recommended. Consumption
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