Cereals and feedstuffs for import and export—Method for the determination of crude fibre content

SN/T 0800.8-1999 Method for testing crude fiber content of grain and feed for import and export SN/T0800.8-1999 Standard download decompression password: www.bzxz.net

This standard is in accordance with GB/T1.1. The experimental research and development standard is corrected after 4. This standard is implemented by the People's Republic of China Eedstuf 93-198 and also replaces the test method for crude fiber content of imported and exported grain and feed. Unit 1: Drafting and expression rules of standards Section 100-1 It is compiled on the basis of "Determination of crude fiber content of crude fiber in export feed" and adopts the method of glass sand core filtration in the Swedish tecator instrument. After repeated submission and coordination by the Entry-Exit Inspection and Quarantine Bureau, this standard drafting unit: Heilongjiang Province, People's Republic of China. The main drafters of this standard: Guo Xiangyun, Xian Kai and Xiao Wu. 1 Scope
Inspection and Quarantine Bureau, Liaoning Entry-Exit Inspection and Quarantine Bureau This standard specifies the determination method of crude fiber content of imported and exported grains and feeds. This standard is applicable to the determination of the total crude fiber content of imported and exported grains and feeds. 2 The referenced standards are the provisions of this standard. They are cited in this standard and constitute the provisions of this standard. SHT0798 9 ​​Inspection of import and export grain, oil and feed Glossary of inspection terms General rules for inspection of import and export grain and feed SHT0800.1-1999 Sampling and sample preparation methods for inspection of import and export grain, oil and feed 18 Inspection methods for moisture and volatile matter in feed SH/T0800.19-1999 3 Definitions This standard adopts the definitions and sample preparation in SH/T0800.1. Test method
601 Sample, first use
5.1 Method principle
basic hair volume, then the test sample is reduced by the standard body stimulation of the pancreas. The disease 5.2 Reagents
as shown in the brand
all standards will be revised, use this standard 10)g sample clean grinder, discard the crushed material. Crush the remaining sample, and the sample that passes through the 1.0m sieve is not less than 95%. Collect the powder and flush the active
05)mc1/L sodium hydroxide standard solution: equivalent to 1.25% (m/m). 5.2.1 (0.313±0.00
5. 2.3.95% ethanol analytical grade)
5. 2.4 Ether (analytical grade)
5.2.5 n-Hexane (analytical grade).
5.3 Instruments and equipment
3.1 Analysis
: Sensitivity port. 1m
5. 3.2 Pulverizer: A sieve plate with an internal aperture of 1.0 mm Digestion container
5.3.4 Oven: Ground-mouth conical flask with a spherical condenser. 5.3.5 Glass sand core: G2, 30mL: F2 made in Sweden 5.3.6 Vacuum filtration device.
5.3.7 Adjustable electric furnace.
5. 3.8 High temperature furnace: (0~1 000)
Desiccant: Contains effective desiccant.
5.4 Analysis steps
Weigh (0.30~3.00)g of sample, which is approximately equivalent to (0.03~0.15)g of relative fiber. The higher the fat content, the more lightly dehydrated it is. Wrap the sample in filter paper and place it in a sugar analyzer; add the acetylcholine of the three layers to the mixture. 5.4.1 Method
5.4.1.1 Acid treatment
contains a container, extracts
, separates, rinses, dries, and condenses
in an oven for about
below the condenser and turn on the heat. Add 20mL of sulfuric acid standard solution that has been boiled in reflux device to the digestion bottle containing the sample, and immediately digest the connection
sand core sugar
5.4.1.2 alkali treatment
and use the human urine sample to weigh the amount of hydrogen peroxide marked in the early filling capsule. The operation is the same as the dangerous treatment method. After heating for 3a, use the reduced pressure condenser to pass through the glandular dense sulfonic acid sand phase filter 5.4.1 3 Solvent washing
Wash the residue three times in a vacuum filtration device bzxz.net
5.4.1.4 Drying and ashing
. Then wash with (2
) mL of ether three times, remove the remaining ether (less than 10% of the sugar content) and take out the obtained sample from a drying chamber for 12 hours and adjust the weight to 0.5 mg: Then perform two parallel tests on the same sample. The method can be used to measure the difference between the sample and the base
5.5 Results
Where: 1-phase fiber content, %
Instrumental method with the same result,
Mass of residue and blockage after drying,
2-Mass of residue and exhaustion after ashing, g: Caloric crude fiber content
Where: 1-phase fiber content
In the sample
Take the arithmetic value of the two measurement results
5.6 Allowable difference
If the total amount of crude fiber is less than 24
Moving mass percentage, %
, the data shall be taken to two decimal places,
should not exceed 5
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