Rearing methods of vector insects used for laboratory efficacy test of public health insecticides for pesticide registration－Part 4:Blattella germanica

NY/T 1964.4-2010 Methods for breeding test insects for indoor tests of registered pesticides Part 4: German cockroaches NY/T1964.4-2010 |tt||Standard download decompression password: www.bzxz.net ||
tt|| This part specifies the breeding methods and control indicators of German cockroaches for indoor efficacy tests of registered pesticides. This part applies to the breeding of German cockroaches for indoor efficacy tests of registered pesticides.

ICS65.100
Agricultural Industry Standard of the People's Republic of China
NY/T1964.4-—2010
Culturing methods of vector insects for laboratory test of public health insecticides registration-
Part 4: Blatella germanica
Published on December 23, 2010
Implemented on February 1, 2011
Ministry of Agriculture of the People's Republic of China
"Culturing methods of vector insects for laboratory test of public health insecticides registration for pesticides" is a series of standards. Part 1: Cicada taeniacus
Part 2: Culex pipiens pallens and Culex pipiens quinquefasciatus
Part 3: Aedes aegypti
Part 1: German mosquito
This part is the first part of the "Indoor test breeding method of public health insecticides for registered drugs". This part was drafted in accordance with the rules of GR/T1.1-2009. NY/T1964.4-2010
This part was proposed and managed by the Ministry of Agriculture of the People's Republic of China. The drafting units of this part are the Pesticide Inspection Institute of the Ministry of Agriculture, Jinan Center for Disease Control and Prevention, Institute of Zoology of the Chinese Academy of Sciences, Institute of Microbiology and Epidemiology of the Chinese Academy of Veterinary Medicine:
The main drafters of this part are: Ben Chunyu, Xin Zheng, Wu Xinping, Li Jiao, Ji Lili, Zhang Hongjie, Nie Xing, 1 Scope
Cultivation methods for indoor test insects of registered pesticides
Part 4: German small algae
NY/T: 1964. 4-2010
This part specifies the cultivation methods and chamber control indicators of German small algae for indoor efficacy tests of registered pesticides. This part applies to the cultivation of German small algae for indoor efficacy tests of registered pesticides. 2 Terms and definitions
The following terms and definitions apply to this document. Cockroach fence cckruachFunce
Refers to a grid structure separation tank made of any material for the breeding of cockroaches. Cynlinder
refers to a device that can separate adult cockroaches from instar cockroaches. 3 Instruments and materials
3.1 Cockroach culture tank: round or square, round, diameter 20cm height ≥30cm; shape, not less than 30cm×H0cm×30cm. 3.2 Cockroach shell: separation distance 1cm~2cm
3.3 Separation tank: fixed round. The bottom should be sealed with a 12-mouth sieve, and the diameter of the sieve should be smaller than the diameter of the round cylinder or the width of the square cylinder. 3.4 Culture blood: diameter 6cm--9cm
3.5 Beaker: 50ml.150ml...
3.6 Compound feed for mice and rats.
3.7 Stone betting Oil.
3.8 Vanadium. Www.bzxZ.net
3.9 Sponge.
4 Rearing conditions
Full temperature: (26±2)℃.
Humidity: (60±10)%
5 Operation steps
5.1 Adults for test
5.1.1 Apply 3cm~~5cm wide oil (paraffin oil: vanadium is 1:1) on the inner edge of the separation site, and put in 2 culture tubes, one containing mouse and rat compound feed, and the other a sponge soaked in water. 5.1.2 Separate and place in the center of the breeding ground with cockroaches at the bottom, and move 30 to 50 cockroaches with brown eggs into the separation tank, and observe and record every day.
NY/T 1964. 4---2010
5.1.3 After the eggs are used up and fermented for 1 day, take out the feed and blood from the separation, shake the separation tank, shake the eggs into the breeding tank, and then transfer the egg-laying adults to the breeding tank, record the separation period, clean the separation tank and utensils, 5.1.4 In the breeding tank, put 2 culture III on the cockroach grid, 1 infected mouse and mouse compound feed, and 1 water-soaked sea cucumber. Replace the water-soaked sponge and supplement the feed every 2 days. You can also use a beaker filled with water to pour water into the culture nozzle. When the water is exhausted, wash the utensils and replace with clean water. 5.1.5 On the 16th day of incubation, kill the unused cockroaches in the experiment, clean the breeding tank and utensils: or transfer them to the adults for subculture. 5.2 Subculture adults
In the breeding tank, put the cockroach grid, and transfer 30 to 50 cockroaches with eggs, or 5.15 200 to 300 adults are placed in 2 culture chambers at the same time, one contains a mixture of feed for mice and rats, and one contains a sponge containing water. Each person replaces the soaked sponge or supplements the feed, and records the feeding process.
6 Control indicators
Adult insects used in the experiment: (100±10) mg/, (50±5) mg/. 7 Precautions
7.1 Paper barriers should be burned when replaced, and other reusable barriers should be disinfected. 7.2 When cleaning the breeding tank, first use hot water to kill the remaining German bugs, and then clean! 7.3 When the insect density in the breeding tank is too high, it should be raised in batches. 7.4 The breeding room should have measures to prevent natural enemies such as spiders, ants, and geckos from entering and the test insects from escaping. 7.5 The breeding room should be kept clean, and cleaning and disposal should be carried out at the end of each generation. Disinfection should be carried out when necessary.
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