Pesticides guidelines for laboratory bioactivity tests Part 3: Soil spray application test for herbicide bioactivity

NY/T 1155.3-2006 Guidelines for Indoor Bioassay Tests for Pesticides Herbicides Part 3: Activity Determination Test Soil Spray Method NY/T1155.3-2006 Standard Download Decompression Password: www.bzxz.net

ICS65.100
Agricultural Industry Standard of the People's Republic of China
NY/T1155.3—2006
Guidelines for Laboratory Bioactivity Tests of Pesticides
Herbicides
Part 3: Activity Test
Soil Spray Method
Pesticides guidelines for laboratory bioactivity testsPart 3: Soil spray application test for herbicide bioactivity2006-07-10 Issued
2006-10-01 Implementation
Ministry of Agriculture of the People's Republic of China
"Guidelines for Laboratory Bioactivity Tests of Pesticides for Herbicides" is a series of standards. This part is the third part of "Guidelines for Laboratory Bioactivity Tests of Pesticides for Herbicides". This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. The drafting unit of this standard: Pesticide Testing Institute of the Ministry of Agriculture. The main drafters of this standard: Zhang Zongjian, Liu Xue, Zhang Hongjun, Zhang Jia, Chen Jie, Wu Zhifeng. This standard is interpreted by the Pesticide Testing Institute of the Ministry of Agriculture. NY/T1155.3—2006
1 Scope
Guidelines for Indoor Bioassay Tests for Pesticides Herbicides Part 3: Activity Determination Test Soil Spray Method This part specifies the basic requirements and methods for the soil spray method to determine the activity of herbicides. NY/T1155.3—2006
This part is applicable to indoor tests for pesticide registration for the determination of the activity of herbicides in soil treatment. Other tests shall be carried out in accordance with this part. 2 Test Conditions
2.1 Test Target
Select representative sensitive weeds that are easy to cultivate and have a consistent growth period, and their seed germination rate should be above 80%. Common weeds are as follows: Grass weeds: Digitaria sanguinalis, Setaria wiridis, Echinochloa crus-galli, Bromus japonicacs, Eleuesine indica, Alopecuarus aequalis, Alopecurus japonicus, Awenafatua, Polypogon fugar, Beckmannia syzigachne, Podanna, etc.
Broadleaf weeds: Abutiton theophrasti, Amaranthus spinosus, Amaranthus retroflerus, Portulaca oleracea, Polygonum aviculare, Descurainia sophia), shepherd's purse (Capsella bursa-pastoris), chenopodium album, wrinkled sorrel (Rumezcrispus), Commelind communis, Xanthium strumarium, Solanum nigrum, Cassiatora, Stellaria media, Stellaria aquatica, Cerastium arvense, Galium umaparine, Veromica didyma, Erigero nannuus, Vicia satiza, Arabidopsis thaliana, etc. Cyperaceae weeds: Cyberusiria, Cyberusrotundus, etc. 2.2 Instruments and equipment
2.2.1 Light incubator or controlled solar greenhouse (light, temperature, humidity, etc.); 2.2.2 Controllable quantitative spray equipment;
2.2.3 Electronic balance (sensitivity 0.1mg); 2.2.4 Pots;
2.2.5 Beaker;
2.2.6 Transfer liquid tube or pipette, etc.
3 Experimental design
3.1 Experimental soil
The test uses air-dried sandy loam with an organic matter content of ≤2%, neutral pH, good permeability, and sieved. 3.2 Test materials
The test soil is quantitatively filled to 4/5 of the pot. The bottom of the pot is used for infiltration irrigation to make the soil completely moist. Evenly spread the pretreated test weed seeds on the soil surface, and then cover the soil with 0.5 cm~2 cm according to the seed size. Spray the soil 24 hours after sowing. 3.3 Agents
3.3.1 Test Agents
The test agent uses the original drug (mother drug) or preparation, and indicates the generic name, trade name or code, content, manufacturer and production date. NY/T1155.3—2006
3.3.2 Control Agents
When the test agent uses the original drug (mother drug), the control agent uses the original drug that has been registered and commonly used in production. The chemical structure type or mode of action of the control agent should be the same or similar to that of the test agent. When the test agent uses a preparation, the control agent uses the original drug of the product and the preparation that has been registered and commonly used in production. 3.4 Experimental steps
3.4.1 Preparation of agents
Water-soluble agents are directly dissolved and diluted with water. Other agents are dissolved in suitable solvents (acetone, dimethylformamide or dimethyl sulfoxide, etc.) and diluted with 0.1% Tween 80 aqueous solution. According to the activity of the agent, 5 to 7 series of doses are set. 3.4.2 Treatment with agents
Calibrate the parameters of the spray equipment (spray pressure and nozzle type), calibrate the spray volume, and spray treatment from low dose to high dose according to the experimental design. Each treatment should be repeated at least 4 times, and a treatment without agents should be set as a blank control. After treatment, move to the greenhouse for routine cultivation and replenish water by infiltration irrigation at the bottom of the pot. Use a temperature and humidity digital recorder to record the dynamic data of temperature and humidity in the greenhouse during the test.
4 Investigation
Regularly observe and record the emergence of weeds and their growth status after emergence after treatment. 14 days or 21 days after treatment, the herbicidal activity was investigated and recorded by visual inspection and absolute value (numerical measurement) survey methods, and damage symptoms such as dwarfing, deformity, and albinism were described. 5 Data statistics and analysis
5.1 Calculation methodwwW.bzxz.Net
5.1.1 Visual inspection method
The herbicidal activity of the agent was evaluated based on the damage symptoms and severity of the test target weeds. The following unified levels can be used for investigation: Level 1: no weeds; Level 2: equivalent to 0-2.5% of the weeds in the blank control area; Level 3: equivalent to 2.6%-5% of the weeds in the blank control area; Level 4: equivalent to 5.1%-10% of the weeds in the blank control area; Level 5: equivalent to 10.1%-15% of the weeds in the blank control area; Level 6: equivalent to 15.1%-25% of the weeds in the blank control area; Level 7: equivalent to 25.1%-35% of the weeds in the blank control area; Level 8: equivalent to 35.1%-67.5% of the weeds in the blank control area; Level 9: equivalent to 67.6%-100% of the weeds in the blank control area. 5.1.2 Absolute value (numerical measurement) investigation method Based on the investigation data, the fresh weight control effect or plant control effect of each treatment is calculated according to formula (1). The unit is percentage (%), and the calculation result is rounded to two decimal places.
EC=Tx100
Wherein:
E——Fresh weight control effect (or plant control effect):
C-Fresh weight of the aboveground part of the control weed (or the number of weed plants);-Fresh weight of the aboveground part of the treated weed (or the number of weed plants). 5.2 Statistical analysis
Use DPS (Data Processing System), SAS (Statistical Analysis System) or SPSS (Statistical Program for Social Sciences) standard statistical software to conduct regression analysis on the logarithm of the amount of the pesticide and the rate of control effect, and calculate the EDso and ED values ​​and 95% confidence limits. 6 Results
NY/T1155.32006
Analyze the test results and evaluate the activity of the pesticide. Combined with the characteristics of the tested pesticides, put forward reasonable suggestions for registering field efficacy trials.
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