GB/T 14926.10-2001 Methods for detecting pathogens in experimental animals

This standard specifies the detection method of Tyzer pathogens in experimental animals. This standard is applicable to the detection of Tyzer pathogens in mice, rats, guinea pigs, hamsters and rabbits. GB/T 14926.10-2001 Detection method of Tyzer pathogens in experimental animals GB/T14926.10-2001 Standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
GB/T 14926.10-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
CE/T14926-10—2001
Non-standard reference is a revision of GB/T14926.10-1994 Laboratory animal test for Bacillus subtilis.
This standard adds ELISA and IFA as optional test methods. This work is issued and approved by the Ministry of Science and Technology of the People's Republic of China. The drafting unit of this standard is China Laboratory Animal Care Association. The main drafter of this standard is Jing Hong. This standard was first issued in January 1994. National Standard of the People's Republic of China. Laboratory Animal Care Method for Detection of Pathogens in Tyeaer's ongassm
ring pathogenic membrane
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GB/T1492
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GB/T14926B
GE/T1492 warehouse
GB/T1492P
GB/T 14926-102001
Representative police GB/T1826-30
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4.8 micropipette, volume 550L, 50200mL, mask with 10 small holes.
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superactive wet box or air conditioning (for preservation of liver), approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on 2001-08-29 and implemented on 2002-05-01
culture Base and test materials
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5.5.1 Specific antigen
GE/T14926.10—2001
Car seriously killed a batch of animals Wangwang PES made of 1,10 space width, cross-talk new process high speed centrifugal supernatant antigen 5.5.2 Normal antigen
Hui more rate Ze soft animal liver, inoculated. .5.1 method. 5.6 Antigen slices 5.6.1 If the animal has serious damage, use the BS method: prepare the slices in an ice container, and ensure that the slices have an appropriate amount of bacteria, that is, the bacteria are not slightly broken, and the fragments are completely opened without overlapping. The whitening phenomenon of the bacteria should be strictly controlled. This should be completed as soon as possible. After the slices are sent to the hospital, they should be solidified for 10 minutes. Rs shake the filled dry end, place it in a 20 preparation bottle,
5.7 drop conjugate
horseradish peroxide labeled sheep or rabbit, too warm, too high, original rat, mouse IgG antibody, use the dry test manufacturer's corresponding animal blood antibodies: loaded with chromatin-labeled sheep anti-rabbit IgG antibody, used to detect immune clear body, Ping silver peroxide foot Teng standard Fan Ming progesterone protein A (5) with dry brush small limit, mouse, rabbit, rabbit please factory fluorescent label sheep or mouse, rat, Phoenix, hamster 1G tray with For the detection of animal specific direct anti-antibodies, 5.8 fluorescent labeled sheep anti-immune serum 1gG, 5.9 positive machine serum, 5.10 SPF blood serum or SPF mouse, rat, mouse, hamster blood serum or non-selective bacterial infection immune serum. 5.11 50% PB5, 5.11 50% glycerol box ... .12 Other trial-made reduced preparations GB/T14926.50-2001 and GB/T14926.52-20016 detection methods
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6-12.1 available specifications note
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GB/T 14926- 102001
subcutaneous
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4-week-old animals can be treated with 200/kg subcutaneous injection, once a month, for 3-5 days, daily. 6-12-2 check
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61 23 color
pressed film and homogenized film were air-dried and fixed with methanol for 5 minutes, then the color was instantly detected and examined with oil microscope. The liver section with punctate necrosis was inoculated with blood (3±13C culture cap for 3 hours, and there was no shadow of nicotinic bacteria. The result was judged by 61-24 hours. The liver pressed film or liver homogenized film should be used for smear. The color was short and hair-like, and the surface was solid with the surface of the smear. Some of the buds could form agar-like surface and the culture showed organoleptic properties. 6.2 Immunoassay (CELISA) 6.2.1 Antibody
Prepare an appropriate amount of working volume, add the effective solution of the antibody and normal antigen respectively, 1 per well, and place it for 1 hour before centrifugation
GB/T 14926 10-2001
6-2.2 Use the selection liquid to make 5 teeth, and then dry the 5mm. 62.3 Add
Test serum and closeness, positive and negative clearing respectively use the interpretation liquid 1140, add to two holes (the foreign antigen hole and the normal antigen hole a100p1h selection net
6-2.4 Add enzyme binding substance
Use the selection liquid to dissolve the compound again into appropriate pressure, air-protected large product core 1, wash the sea as above 6.2.5 Add the bottom drop liquid
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如为抗电男性，
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