Protocol of PCR for detection of genetically modified feed

This standard specifies the qualitative detection method of genetically modified ingredients in feed. SN/T 1201-2003 Qualitative PCR detection method of genetically modified ingredients in plant-based feed SN/T1201-2003 Standard download decompression password: www.bzxz.net
This standard specifies the qualitative detection method of genetically modified ingredients in feed.

People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard SN/T 12012003
Qualitative PCR Detection Method for Genetically Modified Ingredients in Plant-Based Feed
Protocol of PCR for detection of genetically modified feed
Xinmahang
2003-03-17 Issued
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine
2003-09-01 Implementation
This standard is proposed and managed by the National Certification and Accreditation Administration. SN/T12012003
This standard was drafted by Shenzhen Exit-Entry Inspection and Quarantine of the People's Republic of China, and China Import Commodity Inspection Technology Research Institute participated in the drafting.
The main drafters of this standard are Zhang Guiming, Wang, Yu Daojian, Kang Lin, Yang Weidong, Jin Xianzhong, Xi Yinghui, Chen Jinan, Xu Baoliang. This standard is the first published inspection and quarantine industry standard. 1 Model diagram
Qualitative PCR detection method for genetically modified ingredients in plant feed
This standard specifies the qualitative detection method for genetically modified ingredients in feed. SN/T1201-20D3
The qualitative detection method in this standard is applicable to the qualitative detection of genetically modified ingredients in feed made from genetically modified Roundup Ready, genetically modified jade (BT11, BT176, MON810, T14/25, CBH351) and genetically modified oilseed wood (herbicide resistant, herbicide resistant, sterile) as raw materials. 2 Normative references
The clauses in the following documents become the clauses of this standard through reference in this standard. All dated references and their subsequent amendments (excluding errata) and revisions are not applicable to this standard. However, parties to agreements based on this standard are encouraged to study whether the latest versions of these documents can be used. For undated references, the latest versions are applicable to this standard. SN/T0798 Terminology for inspection and testing of imported and exported grains SN/T1193 Laboratory techniques for genetic analysis SN/T1194 Methods for sample preparation and batch testing of genetically modified ingredients in plants and their products Qualitative CR detection method for genetically modified ingredients in rice SN/T 1196
SN/T 1197
Qualitative CR detection method for genetically modified ingredients in rapeseed SN/T1204 Qualitative test method for genetically modified ingredients in plants and their processed products by real-time optical rotation PC:R 3 Terms, definitions and abbreviationsbzxz.net
The following terms, definitions and abbreviations apply to this standard. 3.1 Genetically modified feed (GMF) is a feed made from genetically modified organisms obtained through genetic recombinant technology. 3.2 Polymerase chain reaction (PCR) The template DNA is first denatured into a single strand at high temperature. Under appropriate temperature and in a buffer, two primers anneal to a complementary sequence on the two strands of the template DNA. Then, under the action of DNA synthase, four dNTFs are used as substrates to allow the annealed primers to be synthesized. After repeated cycles of denaturation, annealing and DNA synthesis, the DNA fragment located between the two primer sequences is amplified. 3.3 Qualitative detection Qualitative detection is the detection of genetically modified components in a sample to determine whether the sample is a genetically modified product. 3.4 Abbreviations 3.4. 1GMorgenetically modified organism, genetically modified organism. .3.4.2CaMy35S.35S promoter from tauliflawer ntosnn:virus 35S promoter from tauliflawer ntosnn:virus. SN/T 1201--2D03
3. 4. 3 NOS,termin&tor of nopaline synthase gene from Agrobacterium tumefaciens,
3. 4. 4 CP4 EPSPS:5-enolpyruvylahlkimate-3-phoephate 9ynthase gene,5-fury oxalate-3-iodine synthase gene.3.4.5RRS,RoundupReadyTMSoybean,genetically modified herbicide-resistant product from Monento, USA. 3. 4. 6IVR,Invertase 1 gene from maize+ corn invertase 1 gene.3. 4,7Npt Ⅱ , neomycin-3'-phosphotransferase gene,3. 4, 8 CrylA(b), a synthetile gene encoding the firat 64B amino acids, insecticidal-nctive truncated product identicel to that of cryIA(b) gene af Bacillus thuringiensir subsp, Kurstaki steain HD-l Bacillus thuringiensis crystalline strong protein (Cry) type I toxin insect resistance gene. 4 Source
Qualitative detection of genetically modified feed is to use the difference between foreign genes and plant genes to design primers that can only amplify DNA fragments in foreign genes for PCR amplification. According to the experimental results, it is determined whether the feed contains foreign gene components. 5 Reagents
5. 1 SDS extraction: 1. 5% SDS, EDTA-Na, 100 mmol/L (pH 8. 0), Trig-HCI 20 mmol/L (pH 8, 0), sodium oxide
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