GB 5040-2003 Regulations for quarantine of citrus seedlings at the origin

This standard specifies the types of quarantine pests, seedling cultivation, on-site inspection, indoor inspection, inspection result reporting, epidemic treatment and certification of citrus seedling production areas. This standard is applicable to plant quarantine agencies and all citrus seedling breeding units (individuals) that implement citrus production area quarantine. GB 5040-2003 Citrus seedling production area quarantine regulations GB5040-2003 standard download decompression password: www.bzxz.net

This standard replaces GB5040-2003. Compared with the previous version, this standard has the following changes: GB5040-2003. The revised standard adds the quarantine measures newly announced by the Ministry of Agriculture in 1995 to the list of detectable pests, control measures for pest control agents, identification of pests, etc., adds some new contents and technologies, introduces the international plant quarantine measures on pest-free production areas and pest-free production areas, and keeps the same terms and concepts as those in the relevant international standards. At the same time, some provisions of the previous version of the standard have been appropriately modified. Appendices A and B of this standard are all current appendices. This standard was also issued by the Department of Crop Protection of the Ministry of Agriculture of the People's Republic of China. The originating unit of this standard is: National Agricultural Technology Promotion and Service Center. The drafting units of this standard include the Rural Seedling Inspection Center of the Ministry of Agriculture, Sichuan Provincial Plant Quarantine Station, Hangzhou Quarantine Station of Hubei Province, Jiangxi Provincial Plant Quarantine Station, and Zizhong County Plant Protection Inspection Station of Sichuan Province. The drafters of this standard are Zhao Xueqi, Liu Yuanming, Lin Yun, Zhao Guanluo, and Zhang Dan. This standard is entrusted to the Agricultural Technology Promotion and Service Center of the Ministry of Agriculture. This standard was issued in 1985 and this is the first revision. 1. Country
Origin of Citrus Seedlings Quarantine Procedures
GR5040—2003
This standard specifies the quarantine procedures for citrus production areas, pest species, seedling cultivation, on-site inspection, indoor inspection, inspection result reporting, pest treatment and certification, etc.
This standard applies to plant quarantine agencies and all citrus seedling and seedling management units (individuals) that implement non-citrus production areas. 2 Terms and Definitions
The following terms and definitions apply to regional standards. 2.1
Production points that are separately managed due to plant quarantine. 2.2
Origin Inspection
All quarantine work performed by plant quarantine agencies during the production process at the origin, including field surveys, indoor Inspection, issuing certificates and monitoring the production units, selection and epidemic treatment work, 2.3
Taxes, samples (products) strains or biotypes of plants, animals or pathogens of plant products 2.4
According to epidemic pests
Pests that are economically important but have not yet occurred in the area, or have occurred but are not distributed and are not subject to official control.
3 Symptomatic pests
Citrus Huanglongbing Liberobacteraaaenm (Citrus Hu.uglongbirg) Citrus sticky bacteria Xunthinmuturrispeii (ae)Dye big real plastic Bactrarera (TerradacwinaaEnderlein Bartrocera Tetradacutzsuneonii (Miyeke) Tree thorn small fruit fly Bactraceradarsalis (Headel) No inspection of biological blue wood cultivation
4,1 quarantine declaration
The cultivation unit or individual of citrus seedlings must fill in the origin quarantine application (see Table 1) and can be carried out after review and inspection by the local plant quarantine agency.
GB 5040——2003
Declaration less,
Push object name:
Declaration unit <household):
Special urine point
Special technology plot
Pull the light press machine internal thing model Yizhou.
China #person:
Call music person:
Special value area.
Table 1 Strict land control Jun electric report
Contact number:
For seedlings to ask
Note! : Car table one type one take, the first joint out of the review authority to create the existence, the second family hand in a strange position, note, table set rate unique.
4.2 Selection of seedling site
4.2.2 In areas where Huanglongbing disease occurs, the fenced land must meet one of the following requirements: a) In plain areas, the area shall be more than 1 km from the designated location of the species;
According to the regulations, the estimated number of plants to be planted during the period
and the quantity/kg (sample>
issued by the recipient of the institute of technology
1) In zone 1, there shall be natural barriers such as large rivers and lakes, and no peach or citrus plants within 1.5 km around the area; 4.2.3 In areas where Huanglongbing disease occurs, there shall be no related plants within 1 km around the fenced land. The collection and disinfection of breeding materials
Technical Appendix A,
Establishment of seedling mother garden
Technical Appendix B.
4.5 Prevention measures for seedlings
4.5.| ... 5.1 The plant quarantine personnel shall conduct field inspection after the transfer of the original intention, and before the autumn auction. The fruit of the oranges should be carefully inspected for insects and ants. The indoor inspection shall be carried out after the emergence of the ants. 5.2 Based on the comprehensive daily inspection, the monthly random sampling method shall be used to inspect all seedlings (including 10 sets of sampling): 30% of the seedlings are below 100,000 to 100,000, and 15% of the seedlings are above 100,000). 5.3 If the inspection results are not recorded, the production place will be recorded in detail (see the table). 2
Original unit
Plant species
Cultivation time
Disinfection method
Isolation conditions
Transfer of seedlings
Out-of-house inspection (quarantine-related
occurrence)
Investigation record form for quarantine of origin of fungus wood
Original cultivation site
Initial planting
Surface display quantity
Sparse disease spots
GB5040—2003www.bzxz.net
5.4 When transporting seedlings, all samples below 105 shall be inspected; samples below 1000 shall be inspected at 6%-10%; samples above 11020 shall be inspected at 3%-5%.
6 If quarantine pests are found in samples during direct inspection and it is difficult to diagnose on site, the infested seedlings, diseased specimens or pest standard samples should be promptly sent back to the laboratory for confirmation.
6.2 Identification of quarantined organisms is shown in Appendix 7
Test results
After the inspection of the samples by the Hangzhou Quarantine Laboratory (Control Laboratory), a quarantine report form shall be issued (see Table 3), including the following:
Corresponding application number
Qualification certificate
Inspection method
Inspection summary
Inspector (name)
Reviewer (name)
Sample number
Product name
KANrKA
Sampling period
Location
GB 5040—2003
Epidemic treatment
8.1 If an epidemic animal is found, the animal quarantine agency shall issue a notice of treatment (Form 4) and notify the person in charge to handle it, and the person in charge shall supervise the implementation.
Decline 4 Plant quarantine treatment notice
People
Name of the inspected animal
Infected
Handling opinions
Inspector (character):
Station:
Nature, fine, and handover order group! 8.2 When citrus wilt and Huanglongbing disease occurs, remove the diseased balls immediately, spray pesticides to prevent and control citrus psyllids on the seedling surface and around it, 8.3 When citrus wilt disease plants are found, they should be dug out immediately, and the seedlings should be monitored for 2 to 10 consecutive years in the farming period before autumn and autumn. Use pesticides such as leaf killers to protect the leaves. 8.4 Strictly prohibit the export of seedlings from areas where citrus fruit flies, citrus fruit flies and needle fruit flies occur. After the production place has passed the necessary indoor inspection, if no yellow dragon disease is found, the seedlings and branches of the batch of ... Crop name
seedling quantity
and bidding unit
qualification and quarantine certificate
Table 5 Production withdrawal certificate
variety
field quick effect
producing agency (breeding seal)
variety source
color artist
integration worker
Note 1: The first copy of the pregnancy certificate is exempted from the pulse examination of the breeding unit, and the second copy can be submitted to the quarantine authority. Note 2 This certificate cannot be used for bidding. rkoNrkea
GB 50402003
GD 5Q40—2003
A.1 Seed disinfection
A.1.1 Device
Appendix A
(Consumption specification)
Collection and consumption technology of breeding materials
Ultra-sensitive device 1 piece; bucket, 1 piece: insulation tea bucket, 1 piece; stainless steel, 1 piece; iron mesh cage (or gauze): 1 piece, standard temperature, -1 piece; ordinary temperature.
.1.2 Disinfection steps
A.1.2.1 Add 55~56 hot water in the constant temperature chamber and make it automatically store within 55-0.%, or use a thermos bucket to add a little water to the hot water, and make the water temperature in the chamber reach 55~57 (not higher than 57). A.1.2.2 Put the seeds in a net or gauze bag and place them in 50℃~52 hot water for 5min~6min. .2.3 Take out and put into constant temperature container or heat preservation container immediately. Pay attention to the change of water temperature after planting. Keep the water content within 5℃ + 0.2% for 50min.
A.1.2.4 After treatment, take out and spread out to cool. After disinfection, you can sow or store. A.1.3 Others
Anyone who touches the disinfectant does not need to wash hands with soap. Do not bring any ulcers with you. A.2 Follow-up and disinfection
A, 2. 1. Pick the first
read the purchase. North disease area, old trees in the disease area, and 6-year-old soil disease-free orchards. A.2.2 Disinfection in the south
A.2.2.1 Equipment
Water plug, 1 water tray, 1 plastic tray for disinfection (>1 tray), 2 small magnifying glasses, 70mL volume (or the most appropriate cup), straw paper, A.2.2.2 Disinfection steps
A.2.2.2.1 Check each item under magnification, and the diseased buds should be killed#A.2.2.2.2 Prepare 1000 units/ml of tetracycline solution for standby use , A.2.2.2.3 Put the scion in the dry liquid for 2h, heat it during the process, remove the bubbles on the cut, leaf marks and the limit, rinse it with clean water, and then take it out and transfer it to A.2.2.2.4: A, 2.2.2.4: Put it in the 7 units/1% mixed liquid and take it out for 20mia.~mim. Rinse it with water, push it away, remove the surface moisture, and use disinfectant to protect the packaging from condensation or missing grafting: A.2.3 Others
All personnel who come into contact with the graft must first be washed with water, and the packaging materials must not be infected with bacteria. B. 1% material
Just recorded B
(Normative Appendix)
Maternal scion disinfection technology
GB 504D—2003
Constant temperature sterilization box or sterilization equipment, 1 set of 1 water tank, 1: 200,000 units of each element (531mL), 1: standard thermometer. 15; 1: hygrometer, 1, clean straw paper gauze: magnifying glass, 3. B.2 Sterilization steps
B.2.1 Use magnifying glass to carefully check the sterilization box, remove the buds with diseased spots. B.2.2 Sterilize the sterilization box (add water to the tray). Make the moist and hot air in the box reach 49℃. And keep it at 49℃ for 10.3: B.2.3 Pour the sterilization box into the sterilization box, quickly cover it, and when the temperature rises to 49℃, start calculating the time, and keep it at 49℃ + 0.3: and continue for 50 B2.4 After treatment, transfer the collected liquid into 38~10℃ containing 1% alcohol and 7 units/m2 streptomycin solution (i.e. 10ml needs 1mL ethanol). Stimulate for 30min. B.2.5 After taking out, place it on a clean plate for 20min~30min, rinse, dry the surface moisture, and then store it with gauze for later use. KAONrKAc GB 50402003 C, 1 Citrus Diseases C.1. 1 Symptoms inspection
(Appendix of tobacco standard)
Citrus inspection and identification
Core.1.1.1 Leaf symptoms: The disease ring is initially stuck, the color is convex, the oil is fading, and the disease spots are raised on both sides after expansion, the center is cracked, and the color is spongy, fire white, and then the disease is transformed into a pattern, the surface is reduced, and the color is volcanic and flat. The diseased red home shape cannot be distinguished from the outside. Strictly follow the color rate ring, and the diseased leaves on some leaves are sometimes unclear. C.1.1.2 The diseased spot is almost the same shape, the color surface is fine and solid, there is no color halo, and the diseased spots are connected into irregular patches. Under the condition of explosion, the diseased spots are floating, the water is slightly corroded, raised, the surface is cracked, and the surface is intact + the edge is stained. In resistant varieties, a layer of injured tissue is formed at the junction of the diseased and resistant cultivars. By cutting the outer plug-like substance with a knife and revealing the rough surface, it can be confirmed as an irritant disease. C.1.2 Pathological anatomy
Take a small piece of freshly infected animal and place it at the junction of the diseased area. Drop a drop of secretion water for microscopic examination. If there is a mist-like thick liquid overflowing and a detached layer is formed between the healthy tissues, it can be confirmed as a resistant disease. (.1.3 Pathological adjustment
C.1.3.1 A small piece of infected tissue is rinsed with sterile water and then placed in 1.ml.2.mL. sterile water. Crush the glass with 50% ethanol and keep it in a cool room. Separate the charcoal liquid on the nutrient medium for 2 minutes. Wash with water (100 μl), centrifuge and concentrate, and dilute. Cultivate on artificial medium (20 cells/well), pick out the drops. C:1.3.2 The appropriate culture medium is P. calcite 3.0g, egg yolk (5.0g). 2.5g agar 16.0g) C3.3 The medium is slightly diseased. The shape is bright, the color is shiny, the edge is umbrella-shaped, slightly raised, and sticky. Hydration: The bacteria are organized into chains, with a size of 5-0.7)m×1.F~2.0) and single flagella at both ends, which can move. There is a drop mold, especially piercing sores, single\5 staining medical pieces
C1.4 Detached leaves are isolated from the diseased seedlings in the room, washed with tap water, washed with 1m-1m of amino acid solution on the surface. Rinse thoroughly with sugarcane distilled water under the condition of sterilization, prick the leaves with a needle to make wounds, and place each wound in a culture medium containing 1 head of vegetable flash block (5~11 eyes>10 ~01 active water extract, culture 5-7 large pieces under 25% 30% organic conditions, double frontal needle scratched reaction, usually within a week to form a typical tissue into vertical shape. If private need to do the original isolation method is the same as above.
C.1.5 Serological test
C!1.5. [Antiserum preparation: units confirmed by the final quarantine agency provide standard antiserum disinfection. C.1.5.2 Enzyme absorption test (1.ISA), the unit confirmed by the final quarantine agency provides a diagnostic kit, according to the unified method test: C!.1.5.3 Immunofluorescence ratio1 piece of steel, 1 piece of steel mesh cage (or gauze bag): 1 piece, standard temperature, 1 piece of ordinary temperature.
.1.2 Disinfection steps
A.1.2.1 Add 55~56 hot water to the constant temperature end and make it automatically select and store within 55-0.%, or use a thermos bucket to add a little water to the hot water, and the water temperature in the position is suitable to 55~57 (not higher than 57). A.1.2.2 Put the oranges in a net or gauze bag and put them in 50℃~52 hot water for 5min~6min, 1 .2.3 Take out and put into constant temperature container or heat preservation container immediately. Pay attention to the change of water temperature after planting. Keep the water content within 5℃ + 0.2% for 50min.
A.1.2.4 After treatment, take out and spread out to cool. After disinfection, you can sow or store. A.1.3 Others
Anyone who touches the disinfectant does not need to wash hands with soap. Do not bring any ulcers with you. A.2 Follow-up and disinfection
A, 2. 1. Pick the first
read the purchase. North disease area, old trees in the disease area, and 6-year-old soil disease-free orchards. A.2.2 Disinfection in the south
A.2.2.1 Equipment
Water plug, 1 water tray, 1 plastic tray for disinfection (>1 tray), 2 small magnifying glasses, 70mL volume (or the most appropriate cup), straw paper, A.2.2.2 Disinfection steps
A.2.2.2.1 Check each item under magnification, and the diseased buds should be killed#A.2.2.2.2 Prepare 1000 units/ml of tetracycline solution for standby use , A.2.2.2.3 Put the scion in the dry liquid for 2h, heat it during the process, remove the bubbles on the cut, leaf marks and the limit, rinse it with clean water, and then take it out and transfer it to A.2.2.2.4: A, 2.2.2.4: Put it in the 7 units/1% mixed liquid and take it out for 20mia.~mim. Rinse it with water, push it away, remove the surface moisture, and use disinfectant to protect the packaging from condensation or missing grafting: A.2.3 Others
All personnel who come into contact with the graft must first be washed with water, and the packaging materials must not be infected with bacteria. B. 1% material
Just recorded B
(Normative Appendix)
Maternal scion disinfection technology
GB 504D—2003
Constant temperature sterilization box or sterilization equipment, 1 set of 1 water tank, 1: 200,000 units of each element (531mL), 1: standard thermometer. 15; 1: hygrometer, 1, clean straw paper gauze: magnifying glass, 3. B.2 Sterilization steps
B.2.1 Use magnifying glass to carefully check the sterilization box, remove the buds with diseased spots. B.2.2 Sterilize the sterilization box (add water to the tray). Make the moist and hot air in the box reach 49℃. And keep it at 49℃ for 10.3: B.2.3 Pour the sterilization box into the sterilization box, quickly cover it, and when the temperature rises to 49℃, start calculating the time, and keep it at 49℃ + 0.3: and continue for 50 B2.4 After treatment, transfer the collected liquid into 38~10℃ containing 1% alcohol and 7 units/m2 streptomycin solution (i.e. 10ml needs 1mL ethanol). Stimulate for 30min. B.2.5 After taking out, place it on a clean plate for 20min~30min, rinse, dry the surface moisture, and then store it with gauze for later use. KAONrKAc GB 50402003 C, 1 Citrus Diseases C.1. 1 Symptoms inspection
(Appendix of tobacco standard)
Citrus inspection and identification
Core.1.1.1 Leaf symptoms: The disease ring is initially stuck, the color is convex, the oil is fading, and the disease spots are raised on both sides after expansion, the center is cracked, and the color is spongy, fire white, and then the disease is transformed into a pattern, the surface is reduced, and the color is volcanic and flat. The diseased red home shape cannot be distinguished from the outside. Strictly follow the color rate ring, and the diseased leaves on some leaves are sometimes unclear. C.1.1.2 The diseased spot is almost the same shape, the color surface is fine and solid, there is no color halo, and the diseased spots are connected into irregular patches. Under the condition of explosion, the diseased spots are floating, the water is slightly corroded, raised, the surface is cracked, and the surface is intact + the edge is stained. In resistant varieties, a layer of injured tissue is formed at the junction of the diseased and resistant cultivars. By cutting the outer plug-like substance with a knife and revealing the rough surface, it can be confirmed as an irritant disease. C.1.2 Pathological anatomy
Take a small piece of freshly infected animal and place it at the junction of the diseased area. Drop a drop of secretion water for microscopic examination. If there is a mist-like thick liquid overflowing and a detached layer is formed between the healthy tissues, it can be confirmed as a resistant disease. (.1.3 Pathological adjustment
C.1.3.1 A small piece of infected tissue is rinsed with sterile water and then placed in 1.ml.2.mL. sterile water. Crush the glass with 50% ethanol and keep it in a cool room. Separate the charcoal liquid on the nutrient medium for 2 minutes. Wash with water (100 μl), centrifuge and concentrate, and dilute. Cultivate on artificial medium (20 cells/well), pick out the drops. C:1.3.2 The appropriate culture medium is P. calcite 3.0g, egg yolk (5.0g). 2.5g agar 16.0g) C3.3 The medium is slightly diseased. The shape is bright, the color is shiny, the edge is umbrella-shaped, slightly raised, and sticky. Hydration: The bacteria are organized into chains, with a size of 5-0.7)m×1.F~2.0) and single flagella at both ends, which can move. There is a drop mold, especially piercing sores, single\5 staining medical pieces
C1.4 Detached leaves are isolated from the diseased seedlings in the room, washed with tap water, washed with 1m-1m of amino acid solution on the surface. Rinse thoroughly with sugarcane distilled water under the condition of sterilization, prick the leaves with a needle to make wounds, and place each wound in a culture medium containing 1 head of vegetable flash block (5~11 eyes>10 ~01 active water extract, culture 5-7 large pieces under 25% 30% organic conditions, double frontal needle scratched reaction, usually within a week to form a typical tissue into vertical shape. If private need to do the original isolation method is the same as above.
C.1.5 Serological test
C!1.5. [Antiserum preparation: units confirmed by the final quarantine agency provide standard antiserum disinfection. C.1.5.2 Enzyme absorption test (1.ISA), the unit confirmed by the final quarantine agency provides a diagnostic kit, according to the unified method test: C!.1.5.3 Immunofluorescence ratio1 piece of steel, 1 piece of steel mesh cage (or gauze bag): 1 piece, standard temperature, 1 piece of ordinary temperature.
.1.2 Disinfection steps
A.1.2.1 Add 55~56 hot water to the constant temperature end and make it automatically select and store within 55-0.%, or use a thermos bucket to add a little water to the hot water, and the water temperature in the position is suitable to 55~57 (not higher than 57). A.1.2.2 Put the oranges in a net or gauze bag and put them in 50℃~52 hot water for 5min~6min, 1 .2.3 Take out and put into constant temperature container or heat preservation container immediately. Pay attention to the change of water temperature after planting. Keep the water content within 5℃ + 0.2% for 50min.
A.1.2.4 After treatment, take out and spread out to cool. After disinfection, you can sow or store. A.1.3 Others
Anyone who touches the disinfectant does not need to wash hands with soap. Do not bring any ulcers with you. A.2 Follow-up and disinfection
A, 2. 1. Pick the first
read the purchase. North disease area, old trees in the disease area, and 6-year-old soil disease-free orchards. A.2.2 Disinfection in the south
A.2.2.1 Equipment
Water plug, 1 water tray, 1 plastic tray for disinfection (>1 tray), 2 small magnifying glasses, 70mL volume (or the most appropriate cup), straw paper, A.2.2.2 Disinfection steps
A.2.2.2.1 Check each item under magnification, and the diseased buds should be killed#A.2.2.2.2 Prepare 1000 units/ml of tetracycline solution for standby use , A.2.2.2.3 Put the scion in the dry liquid for 2h, heat it during the process, remove the bubbles on the cut, leaf marks and the limit, rinse it with clean water, and then take it out and transfer it to A.2.2.2.4: A, 2.2.2.4: Put it in the 7 units/1% mixed liquid and take it out for 20mia.~mim. Rinse it with water, push it away, remove the surface moisture, and use disinfectant to protect the packaging from condensation or missing grafting: A.2.3 Others
All personnel who come into contact with the graft must first be washed with water, and the packaging materials must not be infected with bacteria. B. 1% material
Just recorded B
(Normative Appendix)
Maternal scion disinfection technology
GB 504D—2003
Constant temperature sterilization box or sterilization equipment, 1 set of 1 water tank, 1: 200,000 units of each element (531mL), 1: standard thermometer. 15; 1: hygrometer, 1, clean straw paper gauze: magnifying glass, 3. B.2 Sterilization steps
B.2.1 Use magnifying glass to carefully check the sterilization box, remove the buds with diseased spots. B.2.2 Sterilize the sterilization box (add water to the tray). Make the moist and hot air in the box reach 49℃. And keep it at 49℃ for 10.3: B.2.3 Pour the sterilization box into the sterilization box, quickly cover it, and when the temperature rises to 49℃, start calculating the time, and keep it at 49℃ + 0.3: and continue for 50 B2.4 After treatment, transfer the collected liquid into 38~10℃ containing 1% alcohol and 7 units/m2 streptomycin solution (i.e. 10ml needs 1mL ethanol). Stimulate for 30min. B.2.5 After taking out, place it on a clean plate for 20min~30min, rinse, dry the surface moisture, and then store it with gauze for later use. KAONrKAc GB 50402003 C, 1 Citrus Diseases C.1. 1 Symptoms inspection
(Appendix of tobacco standard)
Citrus inspection and identification
Core.1.1.1 Leaf symptoms: The disease ring is initially stuck, the color is convex, the oil is fading, and the disease spots are raised on both sides after expansion, the center is cracked, and the color is spongy, fire white, and then the disease is transformed into a pattern, the surface is reduced, and the color is volcanic and flat. The diseased red home shape cannot be distinguished from the outside. Strictly follow the color rate ring, and the diseased leaves on some leaves are sometimes unclear. C.1.1.2 The diseased spot is almost the same shape, the color surface is fine and solid, there is no color halo, and the diseased spots are connected into irregular patches. Under the condition of explosion, the diseased spots are floating, the water is slightly corroded, raised, the surface is cracked, and the surface is intact + the edge is stained. In resistant varieties, a layer of injured tissue is formed at the junction of the diseased and resistant cultivars. By cutting the outer plug-like substance with a knife and revealing the rough surface, it can be confirmed as an irritant disease. C.1.2 Pathological anatomy
Take a small piece of freshly infected animal and place it at the junction of the diseased area. Drop a drop of secretion water for microscopic examination. If there is a mist-like thick liquid overflowing and a detached layer is formed between the healthy tissues, it can be confirmed as a resistant disease. (.1.3 Pathological adjustment
C.1.3.1 A small piece of infected tissue is rinsed with sterile water and then placed in 1.ml.2.mL. sterile water. Crush the glass with 50% ethanol and keep it in a cool room. Separate the charcoal liquid on the nutrient medium for 2 minutes. Wash with water (100 μl), centrifuge and concentrate, and dilute. Cultivate on artificial medium (20 cells/well), pick out the drops. C:1.3.2 The appropriate culture medium is P. calcite 3.0g, egg yolk (5.0g). 2.5g agar 16.0g) C3.3 The medium is slightly diseased. The shape is bright, the color is shiny, the edge is umbrella-shaped, slightly raised, and sticky. Hydration: The bacteria are organized into chains, with a size of 5-0.7)m×1.F~2.0) and single flagella at both ends, which can move. There is a drop mold, especially piercing sores, single\5 staining medical pieces
C1.4 Detached leaves are isolated from the diseased seedlings in the room, washed with tap water, washed with 1m-1m of amino acid solution on the surface. Rinse thoroughly with sugarcane distilled water under the condition of sterilization, prick the leaves with a needle to make wounds, and place each wound in a culture medium containing 1 head of vegetable flash block (5~11 eyes>10 ~01 active water extract, culture 5-7 large pieces under 25% 30% organic conditions, double frontal needle scratched reaction, usually within a week to form a typical tissue into vertical shape. If private need to do the original isolation method is the same as above.
C.1.5 Serological test
C!1.5. [Antiserum preparation: units confirmed by the final quarantine agency provide standard antiserum disinfection. C.1.5.2 Enzyme absorption test (1.ISA), the unit confirmed by the final quarantine agency provides a diagnostic kit, according to the unified method test: C!.1.5.3 Immunofluorescence ratio1% material
Gang Lu B
(Normative Appendix)
Mother plant and scion disinfection technology
GB 504D—2003
Constant temperature grafting disinfection box or scion disinfection device, 1 unit, 1 water bottle, 1 piece: chain element: 200,000 units: and Qiao (531mL), 1 piece: standard thermometer.15; burial thermometer, 1 piece, clean straw paper gauze: magnifying glass, 3 pieces. B.2 Disinfection steps B.2.1 Check the graft with a magnifying glass and remove the buds with diseased spots. B.2.2 Remove the moisture in the sterilizing box (add water to the tray). Make the hot and humid air in the box reach 49℃ and maintain 49% for 10.3 minutes. B.2.3 Pour the graft into the sterilizing box and quickly cover it. When the temperature rises to 49℃, start calculating the treatment time and maintain 49℃ for 10.3 minutes for 50 minutes. B.2.4 After the treatment, transfer the graft immediately into 38~10℃ containing 1% alcohol 7 units/m2 streptomycin solution (i.e. 10ml needs 1mL ethanol) and stimulate for 30 minutes. B.2.5 Take it out and place it on a clean shelf for 20min~30min, rinse it, dry the surface moisture and then store it with gauze for later use. 50402003
C, 1 Citrus citrus disease
C.1. 1 Symptoms inspection
(Appendix of tobacco standard)
Citrus disease inspection and identification
C.1.1.1 Leaf symptoms: The disease ring is initially stuck, the color is convex, and the oil is fading. After expansion, the disease spots are raised on both sides, the center is broken, and the color is spongy and white. Later, the disease is transformed into a volcanic shape, the surface is reduced, and the color is rotten. The diseased spots are red and cannot be distinguished from other bacteria. Strictly follow the color rate, and the diseased spots on some leaves are sometimes unclear. C.1.1.2 Symptoms: The diseased spots are almost the same shape, the color surface is fine and solid, there is no color halo, and the spots are connected into irregular patches. Under the condition of explosion, the diseased spots are floating. , water slightly cork, bulge, surface rupture, surface integrity + edge rupture. Resistant varieties form a layer of tissue injury at the junction of the disease and the outer surface can be confirmed as irritable disease by cutting the outer plug-like substance with a knife to reveal the rough surface. C, 1, 2 Pathological anatomy || tt || Take a fresh small piece of diseased tissue, and drop a drop of secretion water at the diseased boundary for microscopic examination. If there is a mist-like thick liquid overflowing and a tangled sound is seen, a light separation layer is formed between the healthy tissues, which can be determined as cancer. (. 1.3 Detection || tt || C. 1.3.1 A small piece of diseased tissue is rinsed with sterile water and then placed in 1. ml. 2. mL. sterile water, and sterilized weekly Crush the glass, and keep it in a cool room. Separate the charcoal liquid on the nutrient medium. Wash with water (1U) and centrifuge to concentrate. Cultivate on artificial medium (20 cells or more) and pick out the drops. C:1.3.2 The appropriate culture medium is P. nigra (R, 5.0g egg (I. 2.5g agar 16.0g) C3.3 The microorganisms are in the form of a 5-0.7m × 1.F medium. The microorganisms are in the form of a 5-0.7m × 1.F medium. 2.0) The two ends of the cattle have single flagella, which can move. There is a mold, especially piercing sores, single \ 5 staining medical pieces || tt || C1.4 Detached leaves are isolated from the diseased seedlings in the room, washed with tap water, and the surface was disinfected with 1m-1m of 1x ammonium chloride. Rinse with distilled sugarcane water for 1m-2m. After the leaves are pricked with a needle to cause wounds, each wound (5~11 eyes) is placed in a culture block containing 10~01 active water extract, and cultured for 5-7 people under 25% 30% chemical conditions. The double-sided needle scratched reaction usually forms a typical tissue crack within a week. If it is necessary to do the original separation method is the same as above.
C.1.5 Serological test
C!1.5.【Antisera preparation: The unit confirmed by the final quarantine agency shall provide the standard antisera. C.1.5.2 Enzyme absorption test (ISA), the unit confirmed by the final quarantine agency shall provide the diagnostic kit, and the test shall be performed according to the unified method: C!.1.5.3 Immunofluorescence ratio1% material
Gang Lu B
(Normative Appendix)
Mother plant and scion disinfection technology
GB 504D—2003
Constant temperature grafting disinfection box or scion disinfection device, 1 unit, 1 water bottle, 1 piece: chain element: 200,000 units: and Qiao (531mL), 1 piece: standard thermometer.15; burial thermometer, 1 piece, clean straw paper gauze: magnifying glass, 3 pieces. B.2 Disinfection steps B.2.1 Check the graft with a magnifying glass and remove the buds with diseased spots. B.2.2 Remove the moisture in the sterilizing box (add water to the tray). Make the hot and humid air in the box reach 49℃ and maintain 49% for 10.3 minutes. B.2.3 Pour the graft into the sterilizing box and quickly cover it. When the temperature rises to 49℃, start calculating the treatment time and maintain 49℃ for 10.3 minutes for 50 minutes. B.2.4 After the treatment, transfer the graft immediately into 38~10℃ containing 1% alcohol 7 units/m2 streptomycin solution (i.e. 10ml needs 1mL ethanol) and stimulate for 30 minutes. B.2.5 Take it out and place it on a clean shelf for 20min~30min, rinse it, dry the surface moisture and then store it with gauze for later use. 50402003
C, 1 Citrus citrus disease
C.1. 1 Symptoms inspection
(Appendix of tobacco standard)
Citrus disease inspection and identification
C.1.1.1 Leaf symptoms: The disease ring is initially stuck, the color is convex, and the oil is fading. After expansion, the disease spots are raised on both sides, the center is broken, and the color is spongy and white. Later, the disease is transformed into a volcanic shape, the surface is reduced, and the color is rotten. The diseased spots are red and cannot be distinguished from other bacteria. Strictly follow the color rate, and the diseased spots on some leaves are sometimes unclear. C.1.1.2 Symptoms: The diseased spots are almost the same shape, the color surface is fine and solid, there is no color halo, and the spots are connected into irregular patches. Under the condition of explosion, the diseased spots are floating. , water slightly cork, bulge, surface rupture, surface integrity + edge rupture. Resistant varieties form a layer of tissue injury at the junction of the disease and the outer surface can be confirmed as irritable disease by cutting the outer plug-like substance with a knife to reveal the rough surface. C, 1, 2 Pathological anatomy || tt || Take a fresh small piece of diseased tissue, and drop a drop of secretion water at the diseased boundary for microscopic examination. If there is a mist-like thick liquid overflowing and a tangled sound is seen, a light separation layer is formed between the healthy tissues, which can be determined as cancer. (. 1.3 Detection || tt || C. 1.3.1 A small piece of diseased tissue is rinsed with sterile water and then placed in 1. ml. 2. mL. sterile water, and sterilized weekly Crush the glass, and keep it in a cool room. Separate the charcoal liquid on the nutrient medium. Wash with water (1U) and centrifuge to concentrate. Cultivate on artificial medium (20 cells or more) and pick out the drops. C:1.3.2 The appropriate culture medium is P. nigra (R, 5.0g egg (I. 2.5g agar 16.0g) C3.3 The microorganisms are in the form of a 5-0.7m × 1.F medium. The microorganisms are in the form of a 5-0.7m × 1.F medium. 2.0) The two ends of the cattle have single flagella, which can move. There is a mold, especially piercing sores, single \ 5 staining medical pieces || tt || C1.4 Detached leaves are isolated from the diseased seedlings in the room, washed with tap water, and the surface was disinfected with 1m-1m of 1x ammonium chloride. Rinse with distilled sugarcane water for 1m-2m. After the leaves are pricked with a needle to cause wounds, each wound (5~11 eyes) is placed in a culture block containing 10~01 active water extract, and cultured for 5-7 people under 25% 30% chemical conditions. The double-sided needle scratched reaction usually forms a typical tissue crack within a week. If it is necessary to do the original separation method is the same as above.
C.1.5 Serological test
C!1.5.【Antisera preparation: The unit confirmed by the final quarantine agency shall provide the standard antisera. C.1.5.2 Enzyme absorption test (ISA), the unit confirmed by the final quarantine agency shall provide the diagnostic kit, and the test shall be performed according to the unified method: C!.1.5.3 Immunofluorescence ratio
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