GB/T 5009.49-2003 Analytical methods for hygienic standards of fermented wine

This standard specifies the analysis methods for various sanitary indicators in fermented wine. This standard is applicable to the analysis of various sanitary indicators in wine made from sugar or starch-containing materials, which is not distilled after saccharification and fermentation. GB/T 5009.49-2003 Analysis methods for sanitary standards of fermented wine GB/T5009.49-2003 Standard download decompression password: www.bzxz.net

IC8 67. 040
National Standard of the People's Republic of China
GB/T 5009.49-2003
Generation G300,—s
Method for analysis of hygicnic stnndnrdotfermenledwines
2003-08-11 Issued
2004-01-01 Implementation
Ministry of Health of the People's Republic of China
China Standardization Administration
GD/T5009.49—2003
This standard replaces GB/1ECC9.49
199 European health standard analysis method This standard is different from GB/T500S49-995. The main differences are as follows: This standard is compiled in accordance with B/T20UU1.4·2 (Q1) Standard Preparation Rules Section 4: Chemical Analysis Methods 3 Results of the original standard The determination method of aldehydes in fermented wine was added: This standard was proposed and approved by the Ministry of Health of the People's Republic of China, and the Beijing Municipal Health and Epidemic Prevention Station was responsible for drafting this standard. The Shandong Provincial Health and Epidemic Prevention Station, Liaoning Health Supervision Institute, and Tianjin Municipal Health Supervision Institute were responsible for drafting this standard: The determination method of aldehydes in fermented wine in this standard was mainly initiated by: Lv Qixiang, Xun Yuju, Tu Dantai, Cui Qiming, Liang Jin, Zhang Zhixiazhan. The standard was first issued in 1555 and revised for the first time in 1995. This is the first revision. 2. Scope. tt||Analysis method of hygienic standard of fermented wine. This standard specifies the following items: GR/T5009.49—2003
This standard applies to the analysis of various indicators in raw materials containing or containing substances that have been refined and not smoked.
2 Normative references
The following documents have become the provisions of this standard through reference in this standard: For any referenced document of a certain period, all subsequent versions (excluding the contents of errors) or revisions shall not be used in this standard. However, the latest version of this document may be used according to the opinions of all parties involved in the standard. For referenced documents of a certain period, the latest version shall apply to this standard. GB27 58 Fermented wine health standards
GB: T5009.1% Determination of aluminum in food
GH/T5009.22 Determination of aflatoxin in food GB/T5009.26 Determination of N-amino acids in food GT/T5009.34 Determination of sub-magnesium salts in food CB\5003, H5, 3.1 Take 30mT. of the product, put it in a 50m1 colorless cup, observe its color, it should be transparent without precipitation and impurities. 3.2 Taste it, it should have the unique aroma and flavor of this kind of wine, it should not have any sour, astringent, or strange taste, it should comply with the requirements of GB2758. 4 Physical and chemical tests
According to GB/T5003D.12.
4.2 Aflatoxin
According to GB/T5009.22.
4.3 Distillation
According to GB/T513.34.
If the collected liquor is colored, add salt activated carbon to remove the color, filter, and prepare the filtrate: 4.4 N-acetyl alcohol (beer)
According to GB/T5309.26
4.5 Coloring
According to GB/T5303.35.
4.6 Formaldehyde
4.6.1 Principle
Methylacetone reacts with acetylacetone and 1,4-dihydro-1,5-dihydro-1,6-dihydro-2-nitropropene in the presence of ethyl acetate to form a yellow 3,5-ethylacetone-1,4-dihydro-1,6-dihydro-2-nitropropene compound. There is a yellow absorption at wavelength 1, and the color depth is proportional to the content of formaldehyde, which can be used to determine the content of formaldehyde in the test. 4.6.2 Reagents
4.6.2.1 Dissolve acetylacetone. Weigh 0.4g of acetylacetone and 25g of ammonium acetate. Add 3ml of water and set to 200℃. (Prepare)
GI/T50C9.49—2003
1HHIX.01/1. Thiothioic acid standard solution. 4.6.2.3
0.1ml of standard solution.
4. 6.2. 5
4. 6. 2. 6
4. 6.2. 7
502/1.Starch indicator.
1m/T.Sulfuric acid solution.
Total sodium sulfate solution,
2.298/1.Phosphoric acid solution.
2.6.2.9 Preparation and calibration of aldehyde standard solution: Pipette 3.8% formaldehyde and 7.0% formaldehyde with water to 20% 1, pipette 1U (ml) of this into a 10ml volumetric flask, dilute with water to a sufficient volume. Pipette 10.ml into a 250mL micro-bottle, add 9mm, 0.1ra1/1% nitrate and 15mol/l of sodium iodide, place in a 10mL tower, and add 0.1-mril/1.0L vegetable acid kernels. 1000mol/L sodium aldehyde standard solution is diluted to light color, and 1mT/L is used as indicator. Continue to titrate until blue point appears, and do reagent void test at the same time. The concentration of aldehyde standard solution is calculated according to formula (1): A=VVXX15
Where:
-the volume of aldehyde standard solution is g/mL (mB/mL); V-the volume of insoluble drug in the test sample, in millimoles. The volume of sodium sulfate standard solution consumed in titrating the formaldehyde concentration solution is in milliliters (ml). The concentration of sodium sulfate standard solution is in grams per liter (/-bZxz.net
and 1.) l true standard (:, 00ml/L) is equivalent to the mass of formaldehyde in grams (m). Use the above solution to calibrate the formaldehyde concentration, and water to make a paste of formaldehyde 1/m1. The formaldehyde standard is used quickly: 4.6.3 Instrument required
4.6.3.1 Spectrophotometer | |tt||4.6.3.2 Steam distillation
4.6.3.3500 heating
4.6.4 Analytical steps
4.6.4.1 Sample treatment
Take 35mL of fermented beer with 1% flask containing 200g/L of 200mL of ethanol and steam it, add steam to the steam, add water to dilute the mixture, cool it in a flask (about 1ml), and then dilute it with water. 4.6.4. 2 Determination of the accuracy of the standard 0.00, C.5u. = .00, 2.0, 3004.00.300ml g ml of the standard at 25ml colorimetric water saving rate
absorb the sample increase or decrease 1 and move it into the 85ml colorimetric tube. The standard series and product colorimetric tubes, each containing ? m of the military rate, after mixing, add 10it ohms in boiling water and cool, and then spectrophotometer Determine the absorbance at a wavelength of 120 nm, draw a calibration curve, and derive the sample content from the calibration curve. 4.6.5 Calculation of results
See formula 2):
In the text:
The content of aldehyde in the sample, unit is g (mM): A
The mass of aldehyde equivalent to that determined from the calibration curve, unit is microgram (g): The unit is the sample volume equivalent to that determined from the calibration curve, unit is μg (μL)
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