Pesticides guidelines for laboratory bioactivity tests Part 6: Determining combined action of fungicide mixtures

NY/T 1156.6-2006 Guidelines for Indoor Bioassay Tests for Pesticides Fungicides Part 6: Determination of Combined Action of Mixtures NY/T1156.6-2006 Standard Download Decompression Password: www.bzxz.net

ICS65.100
Agricultural industry standard of the People's Republic of China
NY/T1156.6—2006
Guidelines for laboratory bioactivity tests of pesticides
Fungicides
Part 6: Determining combined action of fungicide mixtures
Pesticides guidelines for laboratory bioactivity testsPart 6: Determining combined action of fungicide mixturesPublished on July 10, 2006
Implemented on October 1, 2006
Ministry of Agriculture of the People's Republic of China
"Guidelines for laboratory bioactivity tests of pesticides - fungicides" is a series of standards. This part is Part 6 of "Guidelines for laboratory bioactivity tests of pesticides - fungicides". This standard is proposed and managed by the Ministry of Agriculture of the People's Republic of China. Drafting unit of this standard: Pesticide Testing Institute of the Ministry of Agriculture. Main drafters of this standard: Zhu Chunyu, Wu Xinping, Xu Wenping, Zhang Wei, Qiu Jianping. This standard is interpreted by the Pesticide Testing Institute of the Ministry of Agriculture. NY/T1156.6—2006
1 ScopewwW.bzxz.Net
Guidelines for Indoor Bioassay Tests of Pesticides Fungicides Part 6: Determination of Combined Effects of Mixtures
This part specifies the basic requirements and methods for the determination of combined effects of mixed fungicides. NY/T1156.6—2006
This part is applicable to indoor tests for pesticide registration to evaluate the combined effects of mixed fungicides. Other tests shall refer to this part. 2 Test conditions
2.1 Test targets
Select the corresponding target pathogens according to the field control objects, and indicate the source of the strains and culture conditions. 2.2 Instruments and Equipment
2.2.1 Electronic balance (sensitivity 0.1mg); 2.2.2
Sprayer;
Microscope:
2.2.4 Pressure steam sterilizer:
Ultra-clean bench;
Biochemical incubator:
Hemacytometer;
3 Pipette or pipette;
2.2.9 Inoculator;
2.2.10 Punch;
2.2.11 Caliper, etc.
3 Experimental Design
3.1 Test Material Preparation
Cultivate the target pathogen on a suitable medium and keep it ready for use. 3.2 Drug
The test drug shall use the original drug (mother drug) and indicate the generic name, trade name or code, content and manufacturer. 3.3 Test steps
3.3.1 Preparation of agents
Water-soluble agents are directly diluted with water. Other agents are dissolved in suitable solvents (methanol, ketone, dimethylformamide or dimethylformamide, etc.) and diluted with 0.1% Tween 80 aqueous solution. Prepare single-dose mother liquors separately, design more than 5 groups of ratios according to the purpose of mixing and the activity of the agent, mix before adding to the culture medium, and set 5 to 7 series of mass concentrations for each single agent and each group of mixed agents. 3.3.2 Agent treatment
According to the different agents and target pathogens, the corresponding treatment methods are adopted. Such as spore germination method, mycelium growth rate method, inhibition zone method, mycelium dry weight method, bacterial suspension turbidity method, etc. For pathogens or viruses that are not suitable for the above methods, the method of indoor seedling or plant tissue inoculation can be used. Each treatment should be repeated at least 3 times, and the treatment without agent should be set as a blank control. 1
NY/T1156.6—2006
4 Investigation
Select the corresponding investigation method according to different test contents and methods. 5 Data statistics and analysis
5.1 Calculation method
Calculate the inhibition rate of the agent on pathogens based on the investigation data. 5.2 Statistical analysis
Use DPS, SAS and SPSS or standard statistical software to perform regression analysis on the logarithmic values ​​of the concentrations of each single agent and different ratios of mixtures and the corresponding inhibition rate values, and calculate the ECs and ECom values ​​and 95% confidence limits. Calculate the synergistic coefficient (SR) or co-toxicity coefficient (CTC) of the mixture according to the Wadley method or Sun Yunpei method. SR≥1.5 indicates a synergistic effect: SR<≤0.5 indicates an antagonistic effect: 0.5
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