GB/T 5009.148-2003 Determination of free gossypol in plant-derived foods

This standard specifies the determination of free gossypol in plant-derived foods by liquid chromatography. This standard is applicable to the determination of free gossypol in vegetable oils or other foods with cottonseed cake as raw materials. The detection limit of this method is 5ng and the minimum detection concentration is 2.5mg/kg. GB/T 5009.148-2003 Determination of free gossypol in plant-derived foods GB/T5009.148-2003 Standard download decompression password: www.bzxz.net

ICSB7.040
National Standard of the People's Republic of China
GB/T5009.148-—2003
代G3/:—998
Determinatier of gossypot fn vegetable foods2003-08-11issued
National Health and Family Planning Commission of the People's Republic of ChinabzxZ.net
Implementation on January 1, 2004
GB/T5099.148—2003
This standard replaces GB/T17334—1999 Determination of novel isocyanate in foods. Compared with GB/T17334-1998, the main features of this standard are as follows: the Chinese name of the standard has been changed to Determination of isocyanate in plant foods; the structure of the original standard has been changed in accordance with GB/T20001.4-2001 Rules for Writing Standards Part 4: Chemical Analysis Methods.
This standard was proposed by the Ministry of Health of the People's Republic of China and the units that formulated this standard are Hebei Provincial Health Inspection and Quarantine Station, Otsu City Food Hygiene Inspection Institute, and Shanxi Provincial Food and Soil Monitoring Institute. The main drafters of this standard are Han Huixin, Jia Li, Chang Fengxin, Tu Quguo and Gao Weiduan. The original standard was issued ten years ago in 1998. This is the second revision. 22
1 Rukun
Determination of free gossypol in plant-based foods
This standard stipulates "The determination of free gossypol in plant-based foods by liquid chromatography. The oil of Changyan is used as the raw material. The determination of gossypol in this standard is based on the method of 5% pure cotton and the minimum detection limit is 1g. 5g/g. 2 Pei Li
GB/T S009.1482003
The free cotton in the oil was extracted with anhydrous water, and the acid was separated from the test sample and measured at 233m. The free cotton in the aqueous test sample was extracted with ethyl acetate and separated by hot water. The impurities were separated and the concentration was determined at 235. The concentration was determined by the external standard method according to the time of the color peak. 3.1. 3.7. 3.3. 3.4. 3.5. 3.6. 3.7. 3.8. 3.9. 4.10. 4.11. 4.12. 4.13. 4.14. 4.15. 4.16. 4.17. 4.18. 4.19. 4.20. 4.21. 4.22. 4.23. 4.24. 4.25. 4.26. 4.27. 4.28. 4.29. 4.30. 4.31. 4.32. 4.33. 4.34. 4.35. 4.36. 4.37. 4.38. 4.39. 4.40. 4.41. 4.42. 4.43. 4.44. 4.45. 4.46. 4.47. 4.48. 4.49. 4.50. 4.51. 4.52. 4.53. 4.54. 4.55. 4.56. 4.67. 4.70. 4.71. 4.72. 4.73. 4.74. 4.75. 4.76. 4.77. 4.78. 4.80. 4.81. This will be equivalent to 1.cms of cotton.
3.7F bottom reduction: absorb 12%/mE cotton phenol reserve concentration 5.1mT. in 1001ml. volumetric bottle, add anhydrous alcohol to make it equal to the amount of cotton. This solution should be used for each cotton.
3. Daily liquid: collect 305 water and add 6.0l to mix 0.5ml, then overfill 4 only
4.1 Liquid phase spectrum: with UV detector: 4.2KD concentration depends on the emission,
4.3 Need to be machined; 3oNr/min
4,413μ[. The amount of injection is %,
4.5Micropark-Cu (250mm-emm). Stainless steel color harmony. 5 analysis steps
5. 1 fish porridge strips
sample flow 40, medium acid flow = 855 determination liquid flow 1mir velvet delivery.25rn/miu: need: 1 sensitivity: 0.At.FS: enter the pen 10l.5.2 test with warning
5.2.1 plant cough gradually sample. 0 people 5 initial anhydrous alcohol dramatic grasp 2mm static blue layer (or refrigerator no), take the upper will wave trace, centrifuge flow as the test structure, liquid phase color. 5.2.2 water tank quality sample, absorb 10.0ml sample. J centrifuge test tube, add 1cnL light water acetylene, select 2min before 5in upper layer 5L, blow with mouse gas, month 1. 0 L seven water ≤ alcohol fixed through, pass through the membrane, as the sample, take 1℃ call. enter the concentrated phase free 253
GB/1 5009,148—2003
Spectrometer:
5.3 Determination
5.3. 1 Preparation of standard line Accurately pipette 0.0, 2.0, 5.0, 8.1 mL of 50 g/mL cottonyphenol standard into a 10.0 mL volumetric medium, dilute to 100 μL with anhydrous ethanol, and add 1, 11, 25, 40 g/L of the standard series. Inject 10 μL as the standard system and plot the standard response values. 5.3.2 Chromatographic analysis: Take 10ul of the sample and inject it into the liquid chromatograph, record the chromatographic brightness and peak height, determine the free phenol content according to the retention time, and calculate the free phenol content from the standard curve according to the peak height. See the following formula: x--the content of phenol in the sample, in milligrams per kilogram (g/kg); a--the content of phenol in the test sample, in micrograms per milliliter (μg/ml); the volume of anhydrous ethanol used, in milliliters (mL). The difference between the results of two precise determinations obtained under repeated measurement conditions shall not exceed 1% of the arithmetic half-mean.
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