GB/T 14926.15-2001 Detection method of Streptococcus pneumoniae in experimental animals

GB/T 14926.15-2001 Detection method of Streptococcus pneumoniae in experimental animals GB/T14926.15-2001 standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
GBT 14926.15-2001
Laboratory animal
Microbiological examination method (2)
Laboratory animalMicrobiological examination method (2) 2001-08-29 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
2002-05-01 Implementation
GB/T14926-15-2001
This standard is a revision of GB/T14926.15-1994 Test Method for the determination of bacterial strains in laboratory animals
This standard adds \6.3.4 Sperm fermentation test and 6.3.5 Opochin positive round test \: It makes a detailed supplement to the content of "5.3.3 Bile salt decomposition test" in GB/T14926-15-1994. This standard is issued by the Ministry of Science and Technology of the People's Republic of China. This standard is issued in January 1944. Chinese Society of Laboratory Animal Science, the main body of the standard
This standard is issued in January 1944.
National Standard of the People's Republic of China
Laboratory Animals
Detection Method of Streptococcus pneumoniae
Laboratory Acid
This standard specifies the experimental animals
This standard is used for laboratory
2 Reference Standards
The following standards are included in the text. Through
For the relevant provisions, all standards refer to
for revision
2001 Experimental Animals
GB/T1492
GG1492
3 Principles
Han Shuangchi Ball
2001 Experimental Head
Baodian of the use of acidbzxz.net
Chemical test, etc., can be distinguished from the same.
Main ingredients and
4.1 Green tea culture medium
4.2 Biological agent
5 Culture medium and reagent
5-1 Grape extract culture medium
Blood glass flat work
53 Serum active medium.
54 Specific alcohol tube.
5.5 Beauty staining solution
56 Grass blue staining solution
10% deoxygenated old pin drop solution.
OptochinF tablets
The main
of this
for use
The General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China approved GB/T on 2001-08-29 14926.15—2001
Daifuxin14925.15—1954
Bangfubai version, the version of the collection
of the new weight card, the weight of the new weight card,
culture base and trial
should be certified, the national highway straight level worker training and production
2002-05-01 implementation
testing procedures||t t||Operation steps
Blood color
GB/T14926-15-2001
±3012h
(364124~h
Yellow grape Hainan set up the culture medium
Uncolored color inspection
Can be made of surface
One night ground
Virtual plane
Option
Deep respiratory tract fluorine secretion substance 7-2 Isolation and culture
7.2-1 Direct isolation and culture
Heavy salt frost
Place the inoculated blood cells in (36±1> for 24-48h for enzyme fermentation
7.2-2 Enhancement culture
Place the inoculated blood cells in (36±1) for 24-48h for enzyme fermentation
7.2-2 Enhancement culture
Place the inoculated blood cells in (36±1) for 12h, then place the inoculated blood cells in (36±1) for 24-48 h.
7.3 Identification room
7.31 Bacterial band
The blood is flat and forms a round shape with a green straight ring around it (blood colony). With the extension of culture time, the colony volume becomes larger due to the self-density effect.
7.3.2 GB/T14925.15-2001
Gram-positive eubacterium, with relatively pointed heads and back-to-back heads, and a pericardium. head mounted, sometimes the inside is cut by Shu row 7.33 Mei Teng staining
the fixed bacteria sample is inoculated with clear quality: 36 1C culture 12 products are carried out Hao Qi Rong color Han Liang Streptococcus in the quantity of the first generation of Mei membrane color national
7.34 Sugar distribution test
Thai teaching first building star positive anti-South
7.35 Optochin Road
pure network culture agent line to be planted on a straight Lang finger flat surface, etc., containing 5, diameter 0p Place the bottle flat on the honey filling surface,
7-36 old salt solution membrane
single straight or 1.0mL of the culture medium is divided into two test tubes, one with 0.5mL of 10% deoxygenated sodium 6.5mL, and the other with 0.5mL of normal saline, and the product is placed in the hand 37 and 35, and it is applied once every 3 hours. If it is positive within 3 hours, the pH must be adjusted to 70 before adding. 7.37 lung girl chain The whole brush of cocci and type A with the ball system is shown in Table 1! With the health of the country and the good nature of
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