GB/T 14926.26-2001 Detection method for mouse encephalomyelitis virus in experimental animals

This standard specifies the detection method and reagents for mouse encephalomyelitis virus (TMEV). This standard is applicable to the detection of mouse TMEV. GB/T 14926.26-2001 Detection method for mouse encephalomyelitis virus in experimental animals GB/T14926.26-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T 14926.26-2001
Laboratory animal
Microbiological examination methods (4)
Laboratory animalMicrobiological examination methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T14926-26-—2001
This standard is a revision of GB 8126—1984 Animal Safety Test Method for the Photoprotection of Mice with Shell 2: The table of examination methods is modified, and only individual text of the original standard is modified. This standard was proposed and drafted by the Ministry of Science and Technology of the People's Republic of China, and the Chinese Society of Laboratory Animal Science was the main drafter of this standard.
This standard was first deposited in January 190.
1 Scope
National Standard of the People's Republic of China
Laboratory animal
Detection method of mouse encephalomyelitis virus
Laboratory animal-Methodrror exindination ofTheiler's mouse ericephalomyelitis virus (TM) This standard specifies the detection method of mouse
This standard is applicable
2 Cited standards
The following standards
are valid. All the provisions of GB/T14 GB/T14526.5 GB/T145 3 original inflammatory disease TMEV will be revised through the revision. According to the immunology products, we can collect "O# TMEV antibody, 4 main reagents and equipment 4-1 trial production 4-11 ELISA antigen 4-1.1.1 specific antigen GB/T14926-262001 agent GB/T14926.26-1994 Shengbo store |At the time of publication of this standard, the versions shown are the most recent.
Use the most recent version of the listed standards when possible. Experimental Animals
Experimental Animals
Use TME
This test can detect the ability of antibodies in serum to detect TMEV (GD strain) infection in mouse serum. After the disease occurs, take the sugar, grind it, and make it into 10% solution. Centrifuge at 3000r/min for 10min, take the supernatant and inoculate BHK21 cells, adsorb for 1.5~2h, add maintenance medium and culture for about 4~5d. When the cell lesion reaches +10~+ ++, and then harvested, thawed three times or ultrasonically treated, and then centrifuged at low speed to remove cell debris. The supernatant was then subjected to ultra-high speed centrifugation to prepare ELTSA antigen. 4.1-1.2 Positive antigen
BHK21 cells were frozen and thawed to break phosphorus, and the supernatant was obtained by low-speed centrifugation to remove cell debris. 41-2 Antigen slice
TMEV (GD bridge) infected BHK21 cells, and cultured for 45 days. When the lesions reached ++~100, the cells were dispersed with trypsin, washed with PBS, and covered. After drying, cold acetone was fixed for 10 minutes and stored at -20°C. Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 200126
2002-05-01wwW.bzxz.Net
4.1.3 Hemoglobin
GB/T14926- 26-2001
Inoculate the brain cavity (O-02mL/mouse) for 14~21d. After 5~7d, take the brain of the mouse aseptically, grind it into 10% suspension 2~3 times, centrifuge at 1000r/min for 10min, add an equal amount of 0.5% deer slurry to the supernatant, and store it at 4C for a short term. 4.1.4 Positive serum
SPF blood serum
4.1.5 Negative serum
SPF mouse serum
4.1.6 Enzyme conjugate
Horseradish peroxide labeled sheep or rabbit anti-mouse IG, horseradish peroxide labeled band required for bacterial protein (ACSPA) 4.1 Isothiomucin fluorescent antibody
American or fish mouse IG antibody
4.2 Equipment
4.2.1 Trademark instrument.
42.2Fluorescent Manwei.
4.23Normal quantity
+2.437C wall ticket
Precision box
Detection method
Positive
Precision result judgment
For positive detector
Result report
According to the judgment result,
( See GB/T14926.52
C See GB/T14926-51
GB/T14926
Carry out serological test
Another kind of screen test.
Use the same Wanyi
Calcium test positive period for life.
1 The most annual shoe wax
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