GB/T 14926.63-2001 Detection method of monkey T lymphocyte tropism virus type Ⅰ in experimental animals

This standard specifies the detection method and reagents for monkey T-lymphocyte tropism virus type 1 (STLV-1). This standard is applicable to the detection of monkey STLV-1. GB/T 14926.63-2001 Detection method for monkey T-lymphocyte tropism virus type Ⅰ in laboratory animals GB/T14926.63-2001 Standard download decompression password: www.bzxz.net

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National Standard of the People's Republic of China
GB/T 14926.63-2001
Laboratory animal
Microbiological examination methods (4)
Laboratory animalMicrobiological examination methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T14926-632001
This standard specifies the detection method for the most effective bacterial tropism toxicity. Simian T Lymphatropism Virus type 1 mainly harms the immune system, causing lesions in immune organs and immune dysfunction. It is a must-check item for pediatric and SPF testing. Therefore, the detection method of this virus is proposed by the Ministry of Science and Technology of the People's Republic of China and is under the jurisdiction of the Chinese Society of Laboratory Animal Science. The main drafter of this standard is Tian Kefei.
National Standard of the People's Republic of China
Laboratory Animal
Laboratory animal-Method for exmination ofSimian T Lymphatropie Virus type 1 (STLV-1)GB/T14926-63-2001
This standard specifies the detection method and reagents of Simian T Lymphatropism Virus type 1 (STLV-1). This standard is applicable to the detection of STLV-1. Cited Standards
The following standards contain provisions that are constitutive of the provisions of this standard through reference in this standard. When this standard is published, the versions indicated are valid. All standards are subject to revision. Parties using this standard should explore the possibility of using the latest version of the following standards. GB/T14926.52-2001
Experimental Animals
Immunofluorescence Test
According to the immunological principle, STLV-1 antigen detection is used to obtain STLV-1 antibodies in serum. Main test materials and equipment
4.1 Reagents
4.1.1 Antigen Slice
HTLV-1 infection case MT4 cells are inoculated for 1014 days and centrifuged at 800t/min for 5-10 minutes, washed with PBS, and smeared. When the net is dried at room temperature, irradiate at 20cm under UV light for 30min, fix with cold acetone for 10min and store at -20℃. 4.1.2 Antisera
Serum obtained from HTLV-1 antigen immunization.
4-1-3 Negative serum
Serum obtained from HTLV-1 and STLV-1 immune immunity.
41.4 Enzyme-conjugated specific
Horseradish peroxide alcohol labeled sheep or rabbit anti-membrane IgG antibody. 4.1.5 Isoacyloxyacid fluorescein labeled sheep or rabbit anti-IgG antibody. 4.2 Equipment
4.2-1 Fluorescence microscope
4.2-237℃ incubator or water bath.
Approved by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China on August 29, 20015
Implemented on May 1, 2002
Testing method
GB/T14926-632001
Use IFA method (see GB/T14926-52-2001) for the detection of virulence. Result determination
For positive test results, use the same method to retest. If it is still positive, it is judged as positive. Result report
Make a report based on the judgment result.
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