GB/T 14926.29-2001 Method for detecting polyomavirus in experimental animals

This standard specifies the detection method and reagents for mouse polyomavirus (POLY). This standard is applicable to the detection of mouse POLY. GB/T 14926.29-2001 Detection method for polyomavirus in experimental animals GB/T14926.29-2001 Standard download decompression password: www.bzxz.net

ICS65.020.30
National Standard of the People's Republic of China
GB/T 14926.29-2001
Laboratory Animals
Microbiological Examination Methods (4)
Laboratory AnimalsMicrobiological Examination Methods2001-08-29Promulgated
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine
2002-05-01Implementation
GB/T14926-292001
This is a revision of the methods for the examination of viruses. No changes were made to the examination methods. This standard is a revision of GB/T14926.29-1994 Laboratory Animals. Only some of the original text was revised. This standard was proposed and managed by the Ministry of Science and Technology of the People's Republic of China. Drafting unit of this standard: China Animal Safety Examination Society Main drafter of this standard: Jie Xiaqin
This standard was first issued in January 1994.
1 Scope
National Standard of the People's Republic of China
Laboratory Animals
Polyoma Virus Detection Method
Laboratory Animal--Methed for Examination of Polyoma Virus (POLY)
This standard specifies mice
This standard is applicable to small rats
2 Reference Standards
The following standards are included
as valid, all standards
GB/T1492
GB/T1492
GB/T1492
3 Principlebzxz.net
of the test
article, through
will be revised, use
2 001 Experimental equipment
Experimental multi-
electrical introduction
corresponding
experimental animal immunofluorescence test
according to the principle of immunology
guarantee
POLY antibody
4 Main test instruments and
4.1 Reagents:
41-1 ELISA antigen C
4-1.1.1 Specific antigen
POLY nucleus strain mouse immunofluorescence
GB/T 14926:292001
Agency GB/T1-926-29-—1994
When the standard is published, the versions shown are the latest versions of the standard.
14+When the cell culture reaches +++~++++, the plate is centrifuged and then made into ELISA antibody. The plate is then centrifuged and then processed.
4.1.1.2 After the ME or 3T3 cell groups are frozen and crushed, they are centrifuged at low speed to remove the supernatant obtained from the drop surface: 4.1.2 Antigen slices, POLY inoculated ME cells, cultured for 10-12 days, when the lesions are suitable, use trypsin to disperse, wash with PBS, smear, dry at room temperature, fix with acetone for 10 minutes, store at -20C, 4.1.3 Positive serum
Antiserum obtained by immunizing SPF mice with POLY antibodies. 4-1.4 Negative serum
SPF mouse blood slides.
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China2001-08-29Approval2002-05-01
4.1.5 Conjugate
GB/T14926-29-2001
Horseradish peroxidase-labeled goat or rabbit anti-mouse IgG antibody: adenocarcinoma peroxidase-labeled phenotypic protein A (SPA). 4.1.6 Fluorescein isothiocyanate labeled sheep or rabbit anti-mouse IgG antibody, 4.2 Equipment
421# label 8
4.2.2 Fluorescence microscope
4.2.3 Sound microscope,
4.2.437C tower culture box or water stagnation box,
5 Detection method
Use ELISA method (see GB/T14926.50-2001) for serum detection, 51
52 Use IFA method (see GB/T1 4926-52-2001》 for blood test. 53
Use IEA method (see GB/T14926-51-2001》 for serological test. 6
Result determination
For positive test results, use the same method or another method to retry. If it is still positive, it is determined to be positive. Result report
Make a report based on the determination result,
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