GB/T 14926.5-2001 Detection method for Pasteurella multocida in experimental animals

GB/T 14926.5-2001 Detection method for Pasteurella multocida in experimental animals GB/T14926.5-2001 Standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
GB/T 14926.5-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GE/T M926.52001
This standard is a revision of GB/T14926.5-1994/Laboratory animalMicrobiological exaration methods). The production of auxiliary hydrogen in cotton is increased by acetic acid. The slide test is mainly for Pasteurella multocida and bronchitis. The "biochemical method of Pasteurella multocida" is not very practical. It was issued by the Ministry of Science and Technology of the People's Republic of China and drafted by Li Hong, the Chinese Society of Zoology. This standard was first revised in January 1994.
1 Yiyuan Www.bzxZ.net
National Standard of the People's Republic of China
Laboratory animal
Test method for Pasteurella multocida
Laboratory animal-Mgihod fer examination This standard is applicable to the test method of experimental animals. It is valid for the test method of the reference sample standard of the standard. All standards are GB/T14986. GB/T14926. 3 principles and principles. This standard is also used to implement the test method of experimental animals. 14926.5-2001
References GB/T14525.5—194
The versions shown at the time of publication are all the new versions of the bioassay.
High and reagents
The positive microbacterium of grass is sent to the upper respiratory tract of rats, hamsters and domestic animals. The specific biochemical points of this book are as follows. The bacteria can be used with the relevant requirements
Please produce grass on the glass slide
4 Main preparations and materials
4.1 General wine plastic culture
4.2 Biological microscope
5 Culture medium and test
Blood gelatin flat top
Dual sugar iron or three-phase sexual store
Sugar love fermentation culture pad
Semi-solid home fat
DHL teaching fat content surface
Element culture medium
Protein stale water, indigo matrix test.
Compound salt culture medium
Baixishi brand brewing salt station culture medium
Nutritional gelatin
Hydrogen acid electric waist response test Xiaotai base.
Oxidation kitchen reagent,
The bacteria are cultured and tested at high temperature,
National Joint Quality Inspection and Quarantine Administration of the People's Republic of China 2001-00-29 Report to LianLE
2002-05-01 5.13 Blood enzyme reagent. 5.14 Pasteurella diagnostic serum, 6 test procedures, 7 steps, 71 sampling, 7-2 elevated wax culture, CH/T-14925.5-2001, 36 minutes of vertical elastic sweet level II (36 main 13 death culture 24~48h73 identification, 100 high-quality certificates, 32 South Korea special surface, GB/T 14926.5-2001
Cao Lan positive Xiao Xuan solid two green garden special ability first, the new separation painting can be installed, after artificial dawn spring want to lose the wine. 7.3.3 Three-cotton lock or double-step culture medium (36±13°C for 18-24°C), the surface and the tiger produce acid but not gas, the lead paper strip shows silicon dioxide positive.
The bacteria are saccharide-positive, milk and hand are positive, the cover alcohol, ornithine degeneration, ammonia alcohol, apple intoxication, nitrate reduction test is positive,
7.3.5 Four Fahrenheit citric acid test, mouse chemistry, hydrogen peroxide is positive, no liquefaction. 7.3.6 The pure culture was transferred to DHL medium. The solid culture medium was kept at 35±13°C for 24-48 hours and no growth was observed.
23 The family wanted to see the liver, the marriage of Pasteur bacteria and bronchitis in the honey of Baogexuan, see Table 1, the capsule 1 multi-soft Pasteur bacteria, Pasteur bacteria and wheat gas electric to the whole table of the air tube transfer relaxation
DHL Enjoy Teng Growth
Help heavy hemolysis
# Solid eye
Acid intake quality
Deer chemical ammonia HS5
Multiple scenes of four Stone bacteria
+Use! —Life—++Big number of bacteria reading! Ten/—Big health number of forest map. Wang Dian Qingshu tablets profit period test positive
Live candy intake report
Any test station that meets the above items will make a positive report, and the non-special report is not a stomach twist. Tiwei
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