GB/T 14926.16-2001 Detection method for beta-hemolytic streptococci in experimental animals

time: 2024-08-09 04:36:17
  • GB/T 14926.16-2001
  • in force

Basic Information

standard classification number

  • Standard ICS number:

    Agriculture>>Agriculture and forestry>>65.020.30 Animal breeding and reproduction
  • China Standard Classification Number:

    Agriculture & Forestry>>Animal Husbandry>>B44 Animal Husbandry

associated standards

Publication information

  • publishing house:

    China Standards Press
  • Publication date:

    2002-05-01

Other Information

  • Release date:

    1994-01-11
  • Review date:

    2004-10-14
  • Drafter:

    He Zhengming
  • Drafting Organization:

    Chinese Society for Laboratory Animal Science
  • Focal point Organization:

    Ministry of Science and Technology of the People's Republic of China
  • Proposing Organization:

    Ministry of Science and Technology of the People's Republic of China
  • Publishing Department:

    Issued by:
  • Competent Authority:

    National Standardization Administration
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Summary:

This standard specifies the detection method of beta-hemolytic streptococci in experimental animals. This standard is applicable to the detection of beta-hemolytic streptococci in mice, rats, guinea pigs, hamsters and rabbits. GB/T 14926.16-2001 Detection method of beta-hemolytic streptococci in experimental animals GB/T14926.16-2001 Standard download decompression password: www.bzxz.net
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Some standard content:

ICS 65.020.30
National Standard of the People's Republic of China
B/T 14926.16-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29 Issued
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GB/T14926:16-2001
This standard amends GB/T14926.16-1994 for the detection of hemorrhagic cocci. It adds a "6.3" biochemical test for the biochemical characteristics of hemorrhagic cocci. Streptococcus aureus test positive\, delete TGB/T14926.181994\5-hand CAMP test\"5-3.4 sodium aesculin test and .5.5% 5-hemolytic acid test" This standard was proposed by the Ministry of Science and Technology of the People's Republic of China, and the drafting unit of this standard is China Academy of Animal Science. This standard was drafted by: Guan, and the standard was first issued in January 1.
National Standard of the People's Republic of China
Laboratory Animals
Detection Method of Type β Hemolytic Streptococcus
Laboratory AnimalsAl-Methid Far Eaminatioa afPhewolyriatregtecocus
This standard specifies the experimental animals for the detection of type β hemolytic streptococci in mice, rats and immune cells. 2 Reference Standards
The following standards are included!
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for teaching. Quality standards need to be favorable, use the car GB/T14926.
GB/T14926.
3 Principle
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experimental animals
experimental animals
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GE/T14926.16— 2001
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and trial production
is the car of Qing Dynasty and the national star life non-charming trial production under the biochemical characteristics, the garden car and biochemical reaction characteristics are adjusted
main equipment and finance
4.1 fragrant temperature culture 0
4.2 biomass micro-marketing.
Year: 3 Henghui car rich box
fine culture medium and reagents
5.1 blood lipid single quantity
5.2 sugar fermentation culture medium.
5.3 Xuanxi fall city tablets.
5# heart tablets#chemical
5.5 infusion culture medium.
5.6 Oxalic acid
People's Republic of China National Quality Supervision and Inspection Standard 2001-08-29 2002-05-01 Implementation
Test procedure
Selection
Can be collected from
GE/T14926.16-2001
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36±15
18-24 h
Blood level
Alarm meat line
18-24h
Presence of typical
fusion batch
7-1 Sampling
Results Take respiratory tract or tumor tissue or organ plan. Qicheng shoes
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There is a joint product test model
7-2 Isolation and culture
Special heart inoculation of blood can be cultured at 7.3 setting
731 Bacterial characteristics
On the blood agar (36 main 13C slightly coupon 18--24h form about 1.0mm, white pattern, raised, half of the night is opaque , smooth surface, 24-round boundary, colorless, bright, straight ring (curtain blood microcolony 7.32 first zero characteristics
Gram graph, spherical or barrel-shaped, straight light 0.%-1.0m, chain arrangement, the first 4 to 8, the longest about 20, the growth on solid culture medium is mostly short or spherical, while in the virus culture medium can form a typical spherical arrangement. 733 positive alcohol test
GR/T 14926.16-2001
Absorb 0.gmL of human blood and add 0.25mL of physiological water, then add 25t36+1C cultured fast-pregnancy culture medium and 0.25mL of 0.25% calcium oxide (calcium oxide has been added, it can be increased to 0.3035%), shake it evenly, place it in a 10mm water container, the blood will condense by itself (put it in the test tube to the degree of agglutination, and the objects inside will not move), and then the blood will be fully dissolved again. If it is completely dissolved after 25 minutes, it is positive. If it is not completely dissolved after 25 minutes, it is the human direct preparation. Oxalic acid 0 minutes of sterilization, then machine 5 people straight, observe the mother, after centrifugation sedimentation, the standard is the grass with the straight case. The child year can be used with || tt || special inspection business pure culture finally book on the plane of Qiongyang, with the usual doctor's intention each piece contains the year of bacillus loading design, yellow on the original fat surface, on (36 life 1 culture 24, rely on the stamped picture to appear as positive Du, still use the known positive trademark as the title, product improvement life dyeing black || tt || fast 17hn registration road device year whole road wax || tt || standard technology grant network .coOm various standards industry information science and technology download
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