GB/T 14926.13-2001 Detection method for Klebsiella pneumoniae in experimental animals

GB/T 14926.13-2001 Detection method of Klebsiella pneumoniae in experimental animals GB/T14926.13-2001 standard download decompression password: www.bzxz.net

ICS 65.020.30
National Standard of the People's Republic of China
BT 14926.13-2001
Laboratory animal
Microbiological exaration methods (2)
Laboratory animalMicrobiological exaration methods2001-08-29Promulgated
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
Implementation on 2002-05-01
GE/T 14926-13-2001
This standard is a revision of GB/T 14926-13-1999 Laboratory animal Microbiological exaration methods. This standard is a revision of GB/T 1492413-1994, 3-155, 3-2, 3-11, 3-3, 3-4, 3-5, 3-6, 3-7, 3-8, 3-9, 4-10, 4-11, 4-12, 4-13, 4-14, 4-15, 4-16, 4-17, 4-18, 4-19, 4-20, 4-21, 4-22, 4-23, 4-24, 4-25, 4-26, 4-27, 4-28, 4-29, 5-30, 5-31, 5-32, 5-33, 5-34, 5-35, 5-36, 5-37, 5-38, 5-39, 6-40, 6-51, 6-6, 6-7, 7-8, 7-9, 8-10, 8-11, 8-12, 8-13, 8-14, 8-15, 8-16, 8-17, 8-18, 8-19, 8-20, 8-21, 8-22, 8-23, 8-24, 8-25
This standard specifies the actual conditions!
This standard applies to mice,
2 Reference standards
The reference standards are valid, all international standards
GB/C13926.
GB/T14926
3 Principle
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Main equipment and materials
Xiang Tong constant temperature culture coupon
5 Culture base and reagents
5.1 DHL class Nanpingwei
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experimental dynamic
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GW/T14926-13-2001
Generation of GB/T14928-13-1990
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base and test
culture medium has the growth, morphology and physiological and biochemical characteristics to be determined 5-2
grams of five weeks Tang Tiewei three top grade
culture enzyme culture medium .
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Rat self-water, indigo matrix reagent
Simon's citrate wax base
Western two-stock drug base
Amino process detoxification test base,
Wu Peisu base.
Semi-solid Korean curtain fat road maintenance,
The General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China approved on August 29, 2001 and implemented on May 1, 2002
6 test procedures
The steps of the procedure
The contents of the ileum are reduced by the ileum, etc.
GB/T14926-1 32001
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7.2 In vitro culture
Place the inoculated DHL culture medium at (36±11C for 24h in a dry cell
7.1 Rong Luo Te Ting
Korean Klebsiella pneumoniae forms colonies on DH monolayer flat blood, light pink, with a large surface and smooth appearance, and is sticky. Some bacteria are fused into a string, and long threads can be pulled out when the inoculation needle is removed. 7.32 Bacterial characteristics
The bacteria are single and have a rod shape, with a size of c0.3-0.5m2/g. They are short and have a beautiful membrane. The inside is single or club-shaped. There is an obvious membrane outside the bacteria. The bacteria can be cultured in the laboratory for 2-3 hours. The membrane disappears after continuous subculture. 7.3.3 This bacterium was transferred to Klebsiella pneumoniae culture medium or 56 soil medium for 18-24 hours. It produces acid on the slope, acid and gas on the bottom layer, but no gas is produced. It has the property of hydrogen precipitation.
7.34 Chemical Identification
VP test positive·salt extract test positive, MR negative, West Palm Press lemon rubber salt application test positive, dimethoate test resistance 4
GB/T 14926-17-2001
, alcohol closure, oxygen-free alcohol positive, food-free limit foot use, Yang bacteria shears and milk grinding, energy matrix use 7.35 Semi-physical dynamic test negative
Standing report
Any person who violates the above inspections will make a positive report, and those who do not meet the requirements will make a sex inference. Membrane
17han carboxylic acid group elimination year static
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